Adhesion-Induced DNA Naturation

Abstract

DNA adsorption and naturation is modeled via two interacting flexible homopolymers coupled to a solid surface. DNA denatures if the entropy gain for unbinding the two strands overcomes the loss of binding energy. When adsorbed to a surface, the entropy gain is smaller than in the bulk, leading to a stronger binding and, upon neglecting self-avoidance, absence of a denatured phase. Now consider conditions where the binding potentials are too weak for naturation, and the surface potential too weak to adsorb single strands. In a variational approach it is shown that their combined action may lead to a naturated adsorbed phase. Conditions for the absence of naturation and adsorption are derived too. The phase diagram is constructed qualitatively.

Locations

• Physical Review Letters - View
• arXiv (Cornell University) - View - PDF
• Wiardi Beckman Foundation (Wiardi Beckman Foundation) - View - PDF
• PubMed - View
• DataCite API - View