DNA Calorimetric Force Spectroscopy at Single Base Pair Resolution
DNA Calorimetric Force Spectroscopy at Single Base Pair Resolution
DNA hybridization is a fundamental reaction with wide-ranging applications in biotechnology. The nearest-neighbor (NN) model provides the most reliable description of the energetics of duplex formation. Most DNA thermodynamics studies have been done in melting experiments in bulk, of limited resolution due to ensemble averaging. In contrast, single-molecule methods have …