Resonant Electro-Optic Imaging for Microscopy at Nanosecond Resolution
Resonant Electro-Optic Imaging for Microscopy at Nanosecond Resolution
We demonstrate an electro-optic wide-field method to enable fluorescence lifetime microscopy (FLIM) with high throughput and single-molecule sensitivity. Resonantly driven Pockels cells are used to efficiently gate images at 39 MHz, allowing fluorescence lifetime to be captured on standard camera sensors. Lifetime imaging of single molecules is enabled in wide-field …