Experimental measurement of binding energy, selectivity, and allostery using fluctuation theorems
Experimental measurement of binding energy, selectivity, and allostery using fluctuation theorems
Thermodynamic bulk measurements of binding reactions rely on the validity of the law of mass action and the assumption of a dilute solution. Yet, important biological systems such as allosteric ligand-receptor binding, macromolecular crowding, or misfolded molecules may not follow these assumptions and may require a particular reaction model. Here …