Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
Abstract Nanosecond temporal resolution enables new methods for wide-field imaging like time-of-flight, gated detection, and fluorescence lifetime. The optical efficiency of existing approaches, however, presents challenges for low-light applications common to fluorescence microscopy and single-molecule imaging. We demonstrate the use of Pockels cells for wide-field image gating with nanosecond temporal …