Reconstruction algorithm for single-particle diffraction imaging experiments
Reconstruction algorithm for single-particle diffraction imaging experiments
We introduce the EMC algorithm for reconstructing a particle's three-dimensional (3D) diffraction intensity from very many photon shot-noise limited two-dimensional measurements, when the particle orientation in each measurement is unknown. The algorithm combines a maximization step $(M)$ of the intensity's likelihood function, with expansion $(E)$ and compression $(C)$ steps that …