Agricultural and Biological Sciences › Insect Science

Bee Products Chemical Analysis

Works Count: 51458

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Description

This cluster of papers focuses on the biological and therapeutic properties of bee products, including propolis, honey, royal jelly, and bee pollen. It explores their antioxidant, antimicrobial, and wound healing capabilities, as well as their chemical composition and botanical origin.

Keywords

Propolis; Honey; Antioxidant; Phenolic Compounds; Royal Jelly; Botanical Origin; Wound Healing; Antimicrobial Activity; Flavonoids; Bee Pollen

Analysis of propolis: some parameters and procedures for chemical quality control

1998-01-01

Ricardo G Woisky , Antônio Salatino

SUMMARYA set of parameters and respective procedures for the establishment of chemical profiles of samples of tinctures and crude propolis is presented. It is proposed that estimations of the content … SUMMARYA set of parameters and respective procedures for the establishment of chemical profiles of samples of tinctures and crude propolis is presented. It is proposed that estimations of the content of total phenolic substances, flavonoids, waxes, ash, volatile substances and dry residue be used as parameters to characterize samples of crude propolis. For tinctures, the estimations of total phenolic substances, flavonoids, waxes, specific gravity and ethanol are proposed. Total phenolic substances and flavonoids are measured by spectrophotometric methods, waxes gravimetrically and ethanol by gas chromatography. The accuracy of the spectrophotometric procedures was tested by assaying a mixture with a known composition of phenolic acids and flavonoids. The use of the procedures is exemplified by the analyses of six samples of crude propolis from different localities in Brazil and of tinctures prepared with absolute and 70% aqueous ethanol. The contents of total phenolic substances, flavonoids, waxes and volatile compounds of the samples analysed are relatively low in comparison with data from the literature. Samples of propolis from nearby localities may have quite different chemical profiles. Compared with absolute ethanol, extraction with aqueous ethanol results in wax-free tinctures, containing higher amounts of phenolic substances.

Honey: Chemical composition, stability and authenticity

2015-09-16

Priscila Missio da Silva , Cony Gauche , Luciano Valdemiro Gonzaga +2 more

The Antibacterial Activity of Honey

1992-01-01

Peter C. Molan

Main European unifloral honeys: descriptive sheets

2004-01-01

Livia Persano Oddo , Roberto Piro

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Antibacterial, antifungal and antiviral activity of propolis of different geographic origin

1999-03-01

A Kujumgiev , Yulia Tsvetkova , Yu Serkedjieva +3 more

Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics

2005-02-01

Giangiacomo Beretta , Paola Granata , Maria Elena Ferrero +2 more

Antioxidant Capacity of Honeys from Various Floral Sources Based on the Determination of Oxygen Radical Absorbance Capacity and Inhibition of in Vitro Lipoprotein Oxidation in Human Serum Samples

2002-04-06

Nele Gheldof , Nicki J. Engeseth

Honeys from seven different floral sources were analyzed for in vitro antioxidant capacity and total phenolic content. Antioxidant capacity was measured by the oxygen radical absorbance capacity (ORAC) assay and … Honeys from seven different floral sources were analyzed for in vitro antioxidant capacity and total phenolic content. Antioxidant capacity was measured by the oxygen radical absorbance capacity (ORAC) assay and by monitoring the formation of conjugated dienes as an index of the inhibition of copper-catalyzed serum lipoprotein oxidation. ORAC values ranged from 3.1 to 16.3 micromol Trolox equivalent/g honey. The darkest colored honeys, such as buckwheat honey, had the highest ORAC values. A linear correlation was observed between phenolic content and ORAC activity of the investigated honeys (p < 0.0001, R (2) = 0.9497). The relationship between the ORAC activity and inhibition of lipoprotein oxidation by the honeys yielded a correlation coefficient of 0.6653 (p = 0.0136). This work shows that honey may be used as a healthy alternative to sugar in many products and thereby serve as a source of dietary antioxidants.

Seasonal effect on Brazilian propolis antibacterial activity

2000-11-01

José Maurício Sforcin , Ary Fernandes Júnior , C. A. Lopes +2 more

A review of the analytical methods to determine the geographical and botanical origin of honey

1998-12-01

Elke Anklam

Functional Properties of Honey, Propolis, and Royal Jelly

2008-11-01

Manuel Viuda‐Martos , Y. Ruiz‐Navajas , Juana Fernández‐López +1 more

ABSTRACT: Honey, propolis, and royal jelly, products originating in the beehive, are attractive ingredients for healthy foods. Honey has been used since ancient times as part of traditional medicine. Several … ABSTRACT: Honey, propolis, and royal jelly, products originating in the beehive, are attractive ingredients for healthy foods. Honey has been used since ancient times as part of traditional medicine. Several aspects of this use indicate that it also has functions such as antibacterial, antioxidant, antitumor, anti‐inflamatory, antibrowning, and antiviral. Propolis is a resinous substance produced by honeybees. This substance has been used in folk medicine since ancient times, due to its many biological properties to possess, such as antitumor, antioxidant, antimicrobial, anti‐inflammatory, and immunomodulatory effects, among others. Royal jelly has been demonstrated to possess numerous functional properties such as antibacterial activity, anti‐inflammatory activity, vasodilative and hypotensive activities, disinfectant action, antioxidant activity, antihypercholesterolemic activity, and antitumor activity. Biological activities of honey, propolis, and royal jelly are mainly attributed to the phenolic compounds such as flavonoids. Flavonoids have been reported to exhibit a wide range of biological activities, including antibacterial, antiviral, anti‐inflammatory, antiallergic, and vasodilatory actions. In addition, flavonoids inhibit lipid peroxidation, platelet aggregation, capillary permeability and fragility, and the activity of enzyme systems including cyclo‐oxygenase and lipoxygenase.

Antioxidant activity of propolis of various geographic origins

2003-07-31

Shigenori Kumazawa , Tomoko Hamasaka , Tsutomu Nakayama

Botanical Origin and Chemical Composition of Brazilian Propolis

2002-03-14

Yong K. Park , Severino Matias de Alencar , Cláudio Lima de Aguiar

Brazilian propolis has been classified into 12 groups based on physicochemical characteristics: five in the southern Brazil group (group 3), one in the southeastern Brazil group (group 12), and six … Brazilian propolis has been classified into 12 groups based on physicochemical characteristics: five in the southern Brazil group (group 3), one in the southeastern Brazil group (group 12), and six in the northeastern Brazil group (group 6). The plant origins of these groups were investigated using reversed-phase high-performance thin-layer chromatography (RPHPTLC), reversed-phase high-performance liquid chromatography (RPHPLC), and gas chromatography−mass spectrometry (GC−MS). It was concluded that the origins of propolis group 3, group 6, and group 12 are resins of the poplar tree, Hyptis divaricata, and Baccharis dracunculifolia, respectively. Keywords: Propolis; flavonoids; Africanized Apis mellifera; bud resin; leaf resin

Contaminants of bee products

2005-12-12

Stefan Bogdanov

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Biological activities and chemical composition of three honeys of different types from Anatolia

2005-12-06

Murat Küçük , Sevgi Kolaylı , Şengül Alpay Karaoğlu +3 more

Caffeic acid phenethyl ester is a potent and specific inhibitor of activation of nuclear transcription factor NF-kappa B.

1996-08-20

Kasthuri Natarajan , Sandeep Singh , Terrence R. Burke +2 more

Caffeic acid phenethyl ester (CAPE), an active component of propolis from honeybee hives, is known to have antimitogenic, anticarcinogenic, antiinflammatory, and immunomodulatory properties. The molecular basis for these diverse properties … Caffeic acid phenethyl ester (CAPE), an active component of propolis from honeybee hives, is known to have antimitogenic, anticarcinogenic, antiinflammatory, and immunomodulatory properties. The molecular basis for these diverse properties is not known. Since the role of the nuclear factor NF-kappa B in these responses has been documented, we examined the effect of CAPE on this transcription factor. Our results show that the activation of NF-kappa B by tumor necrosis factor (TNF) is completely blocked by CAPE in a dose- and time-dependent manner. Besides TNF, CAPE also inhibited NF-kappa B activation induced by other inflammatory agents including phorbol ester, ceramide, hydrogen peroxide, and okadaic acid. Since the reducing agents reversed the inhibitory effect of CAPE, it suggests the role of critical sulfhydryl groups in NF-kappa B activation. CAPE prevented the translocation of the p65 subunit of NF-kappa B to the nucleus and had no significant effect on TNF-induced I kappa B alpha degradation, but did delay I kappa B alpha resynthesis. The effect of CAPE on inhibition of NF-kappa B binding to the DNA was specific, in as much as binding of other transcription factors including AP-1, Oct-1, and TFIID to their DNA were not affected. When various synthetic structural analogues of CAPE were examined, it was found that a bicyclic, rotationally constrained, 5,6-dihydroxy form was superactive, whereas 6,7-dihydroxy variant was least active. Thus, overall our results demonstrate that CAPE is a potent and a specific inhibitor of NF-kappa B activation and this may provide the molecular basis for its multiple immunomodulatory and antiinflammatory activities.

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Identification and Quantification of Antioxidant Components of Honeys from Various Floral Sources

2002-09-10

Nele Gheldof , Xiaohong Wang , Nicki J. Engeseth

Little is known about the individual components of honey that are responsible for its antioxidant activity. The present study was carried out to characterize the phenolics and other antioxidants present … Little is known about the individual components of honey that are responsible for its antioxidant activity. The present study was carried out to characterize the phenolics and other antioxidants present in honeys from seven floral sources. Chromatograms of the phenolic nonpolar fraction of the honeys indicated that most honeys have similar but quantitatively different phenolic profiles. Many of the flavonoids and phenolic acids identified have been previously described as potent antioxidants. A linear correlation between phenolic content and ORAC activity was demonstrated (R(2) = 0.963, p < 0.0001). Honeys were separated by solid-phase extraction into four fractions for sugar removal and separation based on solubility to identify the relative contribution of each fraction to the antioxidant activity of honey. Antioxidant analysis of the different honey fractions suggested that the water-soluble fraction contained most of the antioxidant components. Specific water-soluble antioxidant components were quantified, including protein; gluconic acid; ascorbic acid; hydroxymethylfuraldehyde; and the combined activities of the enzymes glucose oxidase, catalase and peroxidase. Of these components, a significant correlation could be established only between protein content and ORAC activity (R(2) = 0.674, p = 0.024). In general, the antioxidant capacity of honey appeared to be a result of the combined activity of a wide range of compounds including phenolics, peptides, organic acids, enzymes, Maillard reaction products, and possibly other minor components. The phenolic compounds contributed significantly to the antioxidant capacity of honey but were not solely responsible for it.

Honey: its medicinal property and antibacterial activity

2011-04-01

Manisha Mandal , Shyamapada Mandal

Indeed, medicinal importance of honey has been documented in the world's oldest medical literatures, and since the ancient times, it has been known to possess antimicrobial property as well as … Indeed, medicinal importance of honey has been documented in the world's oldest medical literatures, and since the ancient times, it has been known to possess antimicrobial property as well as wound-healing activity. The healing property of honey is due to the fact that it offers antibacterial activity, maintains a moist wound condition, and its high viscosity helps to provide a protective barrier to prevent infection. Its immunomodulatory property is relevant to wound repair too. The antimicrobial activity in most honeys is due to the enzymatic production of hydrogen peroxide. However, another kind of honey, called non-peroxide honey (viz., manuka honey), displays significant antibacterial effects even when the hydrogen peroxide activity is blocked. Its mechanism may be related to the low pH level of honey and its high sugar content (high osmolarity) that is enough to hinder the growth of microbes. The medical grade honeys have potent in vitro bactericidal activity against antibiotic-resistant bacteria causing several life-threatening infections to humans. But, there is a large variation in the antimicrobial activity of some natural honeys, which is due to spatial and temporal variation in sources of nectar. Thus, identification and characterization of the active principle(s) may provide valuable information on the quality and possible therapeutic potential of honeys (against several health disorders of humans), and hence we discussed the medicinal property of honeys with emphasis on their antibacterial activities.

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Recent Advances in the Chemical Composition of Propolis

2014-11-26

Shuai Huang , Cuiping Zhang , Kai Wang +2 more

Propolis is a honeybee product with broad clinical applications. Current literature describes that propolis is collected from plant resins. From a systematic database search, 241 compounds were identified in propolis … Propolis is a honeybee product with broad clinical applications. Current literature describes that propolis is collected from plant resins. From a systematic database search, 241 compounds were identified in propolis for the first time between 2000 and 2012; and they belong to such diverse chemical classes as flavonoids, phenylpropanoids, terpenenes, stilbenes, lignans, coumarins, and their prenylated derivatives, showing a pattern consistent with around 300 previously reported compounds. The chemical characteristics of propolis are linked to the diversity of geographical location, plant sources and bee species.

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Application of microencapsulation in textiles

2002-08-01

G. Nelson

Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity

2004-12-11

Aline Meda , Charles Euloge Lamien , Marco Romito +2 more

Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid)

2005-10-24

İlhami Gülçın

Comparison of the free radical-scavenging activity of propolis from several regions of Argentina

2000-07-01

María I. Nieva Moreno , Marı́a Inés Isla , Antonio Rodolfo Sampietro +1 more

Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey

2007-01-01

Jasna Bertoncelj , Urška DOBERŠEK , Mojca Jamnik +1 more

Propolis: Is there a potential for the development of new drugs?

2010-10-22

José Maurício Sforcin , Vassya Bankova

Phenolic compounds from Brazilian propolis with pharmacological activities

2001-02-01

María Cristina Marcucci , Federico Ferreres , Cristina García‐Viguera +5 more

Propolis: chemical composition, biological properties and therapeutic activity

1995-01-01

María Cristina Marcucci

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Recent progress in pharmacological research of propolis

2001-08-15

Arjun H. Banskota , Yasuhiro Tezuka , Shigetoshi Kadota

Abstract Propolis is a resinous hive product collected by honeybees from various plant sources. It is a popular folk medicine possessing a broad spectrum of biological activities. It has also … Abstract Propolis is a resinous hive product collected by honeybees from various plant sources. It is a popular folk medicine possessing a broad spectrum of biological activities. It has also been used as a health drink in various Asian, European and American countries. Several groups of researchers have focused their attention on the biological activity of propolis and its active principles. Many scientific articles are published every year in different international journals related to the pharmacological properties of propolis. This review article compiles recent findings (since 1995) on the pharmacological properties of propolis focusing on its antihepatotoxic, antitumour, antioxidative, antimicrobial and antiinflammatory properties. The possible mechanism of action of propolis as well as the active compounds are discussed. Copyright © 2001 John Wiley & Sons, Ltd.

Honey for Nutrition and Health: A Review

2008-12-01

Stefan Bogdanov , Tomislav Jurendić , R. Sieber +1 more

Due to the variation of botanical origin honey differs in appearance, sensory perception and composition. The main nutritional and health relevant components are carbohydrates, mainly fructose and glucose but also … Due to the variation of botanical origin honey differs in appearance, sensory perception and composition. The main nutritional and health relevant components are carbohydrates, mainly fructose and glucose but also about 25 different oligosaccharides. Although honey is a high carbohydrate food, its glycemic index varies within a wide range from 32 to 85, depending on the botanical source. It contains small amounts of proteins, enzymes, amino acids, minerals, trace elements, vitamins, aroma compounds and polyphenols. The review covers the composition, the nutritional contribution of its components, its physiological and nutritional effects. It shows that honey has a variety of positive nutritional and health effects, if consumed at higher doses of 50 to 80 g per intake.

Propolis: recent advances in chemistry and plant origin

2000-01-01

Vassya Bankova , Solange L. de Castro , María Cristina Marcucci

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Propolis, an old remedy used in modern medicine

2002-11-01

S. Castaldo , Francesco Capasso

Identification and quantification of methylglyoxal as the dominant antibacterial constituent of Manuka (Leptospermum scoparium) honeys from New Zealand

2008-01-21

Elvira Mavric , Silvia Wittmann , Gerold Barth +1 more

The 1,2-dicarbonyl compounds 3-deoxyglucosulose (3-DG), glyoxal (GO), and methylglyoxal (MGO) were measured as the corresponding quinoxalines after derivatization with orthophenylendiamine using RP-HPLC and UV-detection in commercially available honey samples. Whereas … The 1,2-dicarbonyl compounds 3-deoxyglucosulose (3-DG), glyoxal (GO), and methylglyoxal (MGO) were measured as the corresponding quinoxalines after derivatization with orthophenylendiamine using RP-HPLC and UV-detection in commercially available honey samples. Whereas for most of the samples values for 3-DG, MGO, and GO were comparable to previously published data, for six samples of New Zealand Manuka (Leptospermum scoparium) honey very high amounts of MGO were found, ranging from 38 to 761 mg/kg, which is up to 100-fold higher compared to conventional honeys. MGO was unambigously identified as the corresponding quinoxaline via photodiodearry detection as well as by means of mass spectroscopy. Antibacterial activity of honey and solutions of 1,2-dicarbonyl towards Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were analyzed using an agar well diffusion assay. Minimum concentrations needed for inhibition of bacterial growth (minimum inhibitory concentration, MIC) of MGO were 1.1 mM for both types of bacteria. MIC for GO was 6.9 mM (E. coli) or 4.3 mM (S. aureus), respectively. 3-DG showed no inhibition in concentrations up to 60 mM. Whereas most of the honey samples investigated showed no inhibition in dilutions of 80% (v/v with water) or below, the samples of Manuka honey exhibited antibacterial activity when diluted to 15-30%, which corresponded to MGO concentrations of 1.1-1.8 mM. This clearly demonstrates that the pronounced antibacterial activity of New Zealand Manuka honey directly originates from MGO.

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Antioxidant activities and total phenolics of different types of honey

2002-09-01

Mohamed Al-Mamary , Ali Al‐Meeri , Molham Al‐Habori

A Survey of the Antibacterial Activity of Some New Zealand Honeys

1991-12-01

Kerry L. Allen , P. C. Molan , Gordon McGregor Reid

To assess the variation in antibacterial activity of honey a survey was carried out on 345 samples of unpasteurized honey obtained from commercial apiarists throughout New Zealand. Most of the … To assess the variation in antibacterial activity of honey a survey was carried out on 345 samples of unpasteurized honey obtained from commercial apiarists throughout New Zealand. Most of the honeys were considered to be monofloral, from 26 different floral sources. The honeys were tested against Staphylococcus aureus in an agar well diffusion assay, with reference to phenol as a standard. Antibacterial activity was found to range from the equivalent of less than 2% (w/v) phenol to 58% (w/v) phenol, with a median of 13.6 and a standard deviation of 12.5. Neither the age of the honey samples nor whether they had been processed by the apiarist was associated with lower activity. However, the difference between floral sources in the antibacterial activity was very highly significant. Kanuka (Kunzea ericoides (A. Rich.) J. Thompson. Family: Myrtaceae), manuka (Leptospermum scoparium J. R. et G. Forst. Family: Myrtaceae), ling heather (Calluna vulgaris (L.) Hull. Family: Ericaceae) and kamahi (Weinmannia racemosa Linn. f. Family: Cunoniaceae) were shown to be sources likely to give honey with high antibacterial activity. When antibacterial activity was assayed with catalase added to remove hydrogen peroxide, most of the honeys showed no detectable antibacterial activity. Only manuka and vipers bugloss (Echium vulgare L. Family: Boraginaceae) honeys showed this type of activity in a significant proportion of the samples. The high antibacterial activity of manuka honey was in many cases due entirely to this non-peroxide component.

THE BIOLOGICAL SIGNIFICANCE OF HYALURONIC ACID AND HYALURONIDASE

1947-07-01

Karl Meyer

Review of the biological properties and toxicity of bee propolis (propolis)

1998-04-01

George A. Burdock

Chemical diversity of propolis and the problem of standardization

2005-06-30

Vassya Bankova

Honey Bee Nutrition

1970-01-01

Mykola H. Haydak

With a growing world population and increasingly demanding consumers, the production of sufficient protein from livestock, poultry, and fish represents a serious challenge for the future. Approximately 1,900 insect species … With a growing world population and increasingly demanding consumers, the production of sufficient protein from livestock, poultry, and fish represents a serious challenge for the future. Approximately 1,900 insect species are eaten worldwide, mainly in ...Read More

Propolis and the immune system: a review

2007-05-24

José Maurício Sforcin

Recent trends and important developments in propolis research

2004-12-24

Vassya Bankova

The newest developments in propolis pharmacological research are summarized. The problem regarding biological studies, caused by the chemical variability of propolis, is discussed. The most important trends and developments in … The newest developments in propolis pharmacological research are summarized. The problem regarding biological studies, caused by the chemical variability of propolis, is discussed. The most important trends and developments in recent propolis research are outlined: biological studies performed with chemically characterized samples, bioassay-guided studies of active principles and comparative biological studies of propolis of different origin and chemical composition. These types of studies are extremely valuable with respect to propolis standardization and practical applications in therapy. They will allow scientists to connect a particular chemical propolis type to a specific type of biological activity and formulate recommendations for practitioners.

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Estimation of total flavonoid content in propolis by two complementary colometric methods

2020-07-14

Chu‐Chun Chang , M.-H. Yang , Huiyu Wen +1 more

Honey, Propolis, and Royal Jelly: A Comprehensive Review of Their Biological Actions and Health Benefits

2017-01-01

Visweswara Rao Pasupuleti , Lakhsmi Sammugam , Nagesvari Ramesh +1 more

There are several health benefits that honeybee products such as honey, propolis, and royal jelly claim toward various types of diseases in addition to being food. There are several health benefits that honeybee products such as honey, propolis, and royal jelly claim toward various types of diseases in addition to being food.

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Anti-Inflammatory and Skin Barrier Repair Effects of Topical Application of Some Plant Oils

2017-12-27

Tzu-Kai Lin , Lily Zhong , J.L. Santiago

Plant oils have been utilized for a variety of purposes throughout history, with their integration into foods, cosmetics, and pharmaceutical products. They are now being increasingly recognized for their effects … Plant oils have been utilized for a variety of purposes throughout history, with their integration into foods, cosmetics, and pharmaceutical products. They are now being increasingly recognized for their effects on both skin diseases and the restoration of cutaneous homeostasis. This article briefly reviews the available data on biological influences of topical skin applications of some plant oils (olive oil, olive pomace oil, sunflower seed oil, coconut oil, safflower seed oil, argan oil, soybean oil, peanut oil, sesame oil, avocado oil, borage oil, jojoba oil, oat oil, pomegranate seed oil, almond oil, bitter apricot oil, rose hip oil, German chamomile oil, and shea butter). Thus, it focuses on the therapeutic benefits of these plant oils according to their anti-inflammatory and antioxidant effects on the skin, promotion of wound healing and repair of skin barrier.

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Composition and functional properties of propolis (bee glue): A review

2018-08-17

Syed Ishtiaq Anjum , Amjad Ullah , Khalid Ali Khan +7 more

Propolis is a natural substance collected by honey bees from various plants such as, poplar, palm, pine, conifer secretions, gums, resins, mucilage and leaf buds. It is collected and brought … Propolis is a natural substance collected by honey bees from various plants such as, poplar, palm, pine, conifer secretions, gums, resins, mucilage and leaf buds. It is collected and brought very painstakingly by honey bees to be used for sealing cracks and crevices occurring in their hives. Originally, it as an antiseptic meant for preventing bee-hive from microbial infections along with preventing decomposition of intruders. Additionally, propolis has been used in folk medicine for centuries. The biological characteristics of propolis depend upon its chemical composition, plant sources, geographical zone and seasons. More than 300 compounds have been identified in propolis such as, phenolic compounds, aromatic acids, essential oils, waxes and amino acids. Many scientific articles are published every year in different international journals, and several groups of researchers have focused their attention on the chemical compounds and biological activity of propolis.

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Treatment Strategies for Infected Wounds

2018-09-18

Irina Neguț , Valentina Grumezescu , Alexandru Mihai Grumezescu

The treatment of skin wounds is a key research domain owing to the important functional and aesthetic role of this tissue. When the skin is impaired, bacteria can soon infiltrate … The treatment of skin wounds is a key research domain owing to the important functional and aesthetic role of this tissue. When the skin is impaired, bacteria can soon infiltrate into underlying tissues which can lead to life-threatening infections. Consequently, effective treatments are necessary to deal with such pathological conditions. Recently, wound dressings loaded with antimicrobial agents have emerged as viable options to reduce wound bacterial colonization and infection, in order to improve the healing process. In this paper, we present an overview of the most prominent antibiotic-embedded wound dressings, as well as the limitations of their use. A promising, but still an underrated group of potential antibacterial agents that can be integrated into wound dressings are natural products, especially essential oils. Some of the most commonly used essential oils against multidrug-resistant microorganisms, such as tea tree, St. John’s Wort, lavender and oregano, together with their incorporation into wound dressings are presented. In addition, another natural product that exhibits encouraging antibacterial activity is honey. We highlight recent results of several studies carried out by researchers from different regions of the world on wound dressings impregnated with honey, with a special emphasis on Manuka honey. Finally, we highlight recent advances in using nanoparticles as platforms to increase the effect of pharmaceutical formulations aimed at wound healing. Silver, gold, and zinc nanoparticles alone or functionalized with diverse antimicrobial compounds have been integrated into wound dressings and demonstrated therapeutic effects on wounds.

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Phenolic Compounds in Honey and Their Associated Health Benefits: A Review

2018-09-11

Danila Cianciosi , Tamara Y. Forbes‐Hernández , Sadia Afrin +7 more

Honey is a natural substance appreciated for its therapeutic abilities since ancient times. Its content in flavonoids and phenolic acids plays a key role on human health, thanks to the … Honey is a natural substance appreciated for its therapeutic abilities since ancient times. Its content in flavonoids and phenolic acids plays a key role on human health, thanks to the high antioxidant and anti-inflammatory properties that they exert. Honey possesses antimicrobial capacity and anticancer activity against different types of tumors, acting on different molecular pathways that are involved on cellular proliferation. In addition, an antidiabetic activity has also been highlighted, with the reduction of glucose, fructosamine, and glycosylated hemoglobin serum concentration. Honey exerts also a protective effect in the cardiovascular system, where it mainly prevents the oxidation of low-density lipoproteins, in the nervous system, in the respiratory system against asthma and bacterial infections, and in the gastrointestinal system. A beneficial effect of honey can also be demonstrated in athletes. The purpose of this review is to summarize and update the current information regarding the role of honey in health and diseases.

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Antibacterial Properties of Propolis

2019-05-29

Izabela Przybyłek , Tomasz M. Karpiński

Researchers are continuing to discover all the properties of propolis due to its complex composition and associated broad spectrum of activities. This review aims to characterize the latest scientific reports … Researchers are continuing to discover all the properties of propolis due to its complex composition and associated broad spectrum of activities. This review aims to characterize the latest scientific reports in the field of antibacterial activity of this substance. The results of studies on the influence of propolis on more than 600 bacterial strains were analyzed. The greater activity of propolis against Gram-positive bacteria than Gram-negative was confirmed. Moreover, the antimicrobial activity of propolis from different regions of the world was compared. As a result, high activity of propolis from the Middle East was found in relation to both, Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) strains. Simultaneously, the lowest activity was demonstrated for propolis samples from Germany, Ireland and Korea.

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Propolis: A Review

1979-01-01

E.L. Ghisalberti

Progress in the Chemistry of Organic Natural Products 109

2019-01-01

A. Douglas Kinghorn , Heinz Falk , Simon Gibbons +3 more

Protective Effects of Aqueous Propolis Extract from the Artvin-Hatila Region of Türkiye Against Zinc Oxide-Induced Genotoxicity: Antimutagenic, Antioxidant, and Phytochemical Evaluation

2025-06-24

Nurşen Aksu Kalmuk | Muş Alparslan Üniversitesi Fen Bilimleri Dergisi

This study investigated the protective effects of an aqueous propolis extract obtained from the Artvin-Hatila region of Türkiye against genotoxic stress induced by zinc oxide (ZnO) exposure in Allium cepa … This study investigated the protective effects of an aqueous propolis extract obtained from the Artvin-Hatila region of Türkiye against genotoxic stress induced by zinc oxide (ZnO) exposure in Allium cepa root meristem cells. Cytogenetic parameters such as mitotic index, chromosomal abnormalities, and micronucleus formation were evaluated. Antioxidant capacity was measured using DPPH, FRAP, and CUPRAC assays. The phenolic compound profile was determined via HPLC, and total phenolic and flavonoid contents were spectrophotometrically quantified. The results demonstrated that the 100 mg/L concentration of propolis significantly reduced ZnO-induced cytogenetic damage. At both lower and higher concentrations, this effect was diminished, and genotoxicity increased in some parameters. The findings suggest that the phenolic compounds in propolis may suppress ZnO-related genotoxic stress and, when applied within an appropriate dose range, contribute to maintaining cellular structural integrity in biological systems. These results indicate that propolis may represent a promising natural resource for the development of alternative protective strategies against environmental toxic agents.

Overview of bee research in Roraima, Brazil

2025-06-24

Edymeiko de Souza Maciel , Gardênia Holanda Cabral | Revista Agro mbiente On-line

Bees are fundamental to biodiversity conservation, as they are indispensable pollinators and contribute significantly to the economy, especially in the agricultural sector. However, there are still many knowledge gaps regarding … Bees are fundamental to biodiversity conservation, as they are indispensable pollinators and contribute significantly to the economy, especially in the agricultural sector. However, there are still many knowledge gaps regarding species diversity and the benefits provided by bees, including their products, particularly in the Northern region of Brazil. In this context, this research sought to provide an overview of beerelated research based on studies conducted in the state of Roraima that used bees and/or their products as the central theme. Searches were carried out in SciELO, Google Scholar, PubMed, the CAPES Journal Portal, the Brazilian Digital Library of Theses and Dissertations and the institutional repositories of the Federal University of Roraima and the State University of Roraima. A total of 43 studies were analyzed, including scientific articles, theses, and dissertations that studied bees and/or their products. Most of the studies were scientific journal articles (60%), followed by dissertations (28%), while books, book chapters, and theses appeared in smaller numbers, 5%, 5%, and 2%, respectively. The most studied fields were apiculture (35%) and physicochemical and microbiological analysis of apicultural products (26%). These findings provide valuable insights into the understanding of bees in Roraima and highlight the discrepancy between the importance of the topic and the number of studies conducted in the state.

Evaluation of the Antimicrobial Activity of Propolis Extracts from Family Farming

2025-06-24

Thais Mendes Ramos , Fabrícia Vieira Silva Bomtempo , Eskálath Morganna Silva Ferreira +2 more | Revista de Gestão Social e Ambiental

Objective: To evaluate the antimicrobial efficacy of propolis extracts derived from family farming against pathogens associated with oropharyngeal, gastrointestinal, and skin infections, as well as dental caries and candidiasis. Theoretical … Objective: To evaluate the antimicrobial efficacy of propolis extracts derived from family farming against pathogens associated with oropharyngeal, gastrointestinal, and skin infections, as well as dental caries and candidiasis. Theoretical Framework: Beekeeping, an economically viable and environmentally strategic activity, can be sustainably carried out in Permanent Preservation Areas and Legal Reserves, promoting biodiversity conservation, native species pollination, and the strengthening of ecologically based production systems. Additionally, propolis—one of its main by-products—can contribute to production diversification, income generation, and the development of products with therapeutic and cosmetic applications. Method: An initial screening of the antimicrobial activity of ethanolic propolis extracts (one commercial and three from family farming) was conducted using the well diffusion method, with antimicrobial activity assessed by measuring inhibition halos. Subsequently, broth microdilution assays were performed in 96-well microplates to determine the Minimum Inhibitory Concentration (MIC) of each extract. Results/Discussion: The tested extracts inhibited the growth of the evaluated microorganisms and presented satisfactory MICs when compared to the commercial extract. Research Implications: The laboratory analysis provides practical contributions to the bioeconomy by strengthening beekeeping as a driver of socio-environmental development. Originality/Value: This study highlights the promising potential of propolis extracts in controlling both bacterial and fungal infections, thereby promoting the valorization of sustainable production systems, contributing to production diversification, and reinforcing the local economy.

Investigation of the Gastroprotective Activity of Propolis Extracted in Different Solvents

2025-06-24

Ali Sorucu , Osman Bulut , Ayşe Nur Akkoç +1 more | Animal Health Production and Hygiene

Propolis is a product produced by honeybees that collects the resins of plants. Secondary metabolites, including phenolic compounds, which are found in the resin of plants and are incorporated into … Propolis is a product produced by honeybees that collects the resins of plants. Secondary metabolites, including phenolic compounds, which are found in the resin of plants and are incorporated into propolis, give propolis many biological properties. Propolis exhibits various properties such as antibacterial, antiviral, antifungal, antioxidant, immunomodulator, anti-inflammatory, antiulcer and wound healing accelerator. The properties vary depending on the source of the plant, season, altitude, climate zone and extraction solvent. The present study investigated and compared the effects of different propolis extracts prepared using alcohol, water, dimethyl sulfoxide (DMSO), and olive oil on the indomethacin-induced gastric ulcers in nine group of Balb/C mice (n=8). Epithelial loss, erosion, bleeding, edema, inflammatory cell infiltration, and mean clinical scores results were evaluated statistically between different propolis extracts compared to omeprazole. Since the histopathological results were remarkably similar, the general assessment was made with the mean clinical score. It was found that DMSO and olive oil extracts of propolis had gastroprotective effects similar to omeprazole. In contrast, hydro-alcohol and water extracts did not show significant differences compared to their solvents and gastroprotective activity. In conclusion, it was determined that the olive oil extract of propolis, which is especially suitable for direct consumption, has the potential to be used as a gastroprotective.

Characterizing pollen profiles in honey from Melipona quadrifasciata anthidioides : a melissopalynological study

2025-06-23

Natália Sêneda Martarello , Adriana de Oliveira Fidalgo , Cynthia Fernandes Pinto da Luz | Palynology

Uji Efektivitas Sediaan Nanoparticle Lipid Carrier (NLC) Kombinasi Ekstrak Teh Hijau dan Asam Alfa-Lipoat sebagai Antiaging

2025-06-23

Abdurrahman Abdurrahman , Fairuz Yaumil Afra , Widji Soeratri +1 more | Journal of Pharmaceutical And Sciences

Latar Belakang: Asam alfa-lipoat berperan sebagai ko-antioksidan yang berpotensi meningkatkan efektivitas ekstrak teh hijau sebagai agen antiaging dalam sistem Nanostructured Lipid Carrier (NLC) dengan meningkatkan efisiensi penjebakannya. Kombinasi ini diharapkan … Latar Belakang: Asam alfa-lipoat berperan sebagai ko-antioksidan yang berpotensi meningkatkan efektivitas ekstrak teh hijau sebagai agen antiaging dalam sistem Nanostructured Lipid Carrier (NLC) dengan meningkatkan efisiensi penjebakannya. Kombinasi ini diharapkan dapat meningkatkan manfaat antiaging melalui peningkatan kerapatan kolagen dan jumlah fibroblas pada kulit. Tujuan: Penelitian ini bertujuan untuk mengetahui efektivitas sediaan NLC kombinasi ekstrak teh hijau dan asam alfa-lipoat sebagai agen antiaging. Metode: Sistem NLC dibuat menggunakan metode high-shear homogenization dengan tiga formula: F1 (tanpa asam alfa-lipoat), F2 (1% asam alfa-lipoat), dan F3 (1,5% asam alfa-lipoat). Uji efektivitas dilakukan secara in vivo menggunakan mencit dengan parameter pengamatan berupa kerapatan kolagen dan jumlah fibroblas pada kulit punggung mencit selama 14 hari. Hasil Penelitian: Hasil menunjukkan bahwa formula F2 dan F3 memiliki kerapatan kolagen dan jumlah fibroblas yang lebih tinggi secara signifikan dibandingkan F1, menunjukkan bahwa penambahan asam alfa-lipoat meningkatkan efektivitas antiaging. Kesimpulan: Penelitian ini membuktikan bahwa sediaan NLC kombinasi ekstrak teh hijau dan asam alfa-lipoat efektif dalam meningkatkan kerapatan kolagen dan jumlah fibroblas, sehingga berpotensi sebagai agen antiaging yang lebih baik dibandingkan tanpa penambahan ko-antioksidan.

Potencial terapêutico de Egletes viscosa (L.) less: uma revisão sistemática

2025-06-23

Bárbara Orrana Sobreira da Silva , A. Amariz , Melquisedec de Sousa Oliveira +1 more | Saúde e meio ambiente revista interdisciplinar

In Brazil, research with medicinal plants grew complete, mainly focused on the area of pharmacology, with application in several areas of health. Several studies have evidenced the capacity of medicinal … In Brazil, research with medicinal plants grew complete, mainly focused on the area of pharmacology, with application in several areas of health. Several studies have evidenced the capacity of medicinal plants for the development and supply of new drugs. Among these species, the Egletes viscosa (L.) Less, a promising botanical species, used mainly in the Brazilian Northeast, with therapeutic potential used in popular medicine. In this sense, the objective of this work was to compile the studies that reported the therapeutic potential of Egletes viscosa. It is used as electronic databases PubMed, Web of Science, SciELO and capes journals. The research was carried out between September and December 2021, the keywords were in Portuguese and English, using the following terms: Egletes viscosa or Macela, with the Boolean operator “or”. 217 publications distributed in the databases were identified, 72 duplicate articles were excluded. Only 24 articles were found in relation to the theme and were selected for reading the abstract/abstract. Of these, 21 articles were selected for full reading. After evaluation and reading, the study focused on 13 articles. In general, it was found that this vegetable has antibiotic, antinoceptive, antidermatitis, antidepressant, anxiolytic, anticancer, analgesic, gastroprotective, anti-inflammatory, anti-diarrheal, anti-anaphylactic, anti-thrombotic and anti-hepatotoxic activities. Its compounds can be used in the development of herbal medicines, however, studies are still underway. Key words: medicinal plants; macela; phytotherapy.

Behind the Therapeutic Effects of Royal Jelly: Recent Advances in the Specific Properties of 10-Hydroxydecanoic Acid

2025-06-22

Carla Gasbarri , Guido Angelini | Molecules

Since ancient times, Royal Jelly (RJ) has been known for its remarkable properties in traditional medicine, and it is still widely recommended for mental and physical well-being. RJ consists of … Since ancient times, Royal Jelly (RJ) has been known for its remarkable properties in traditional medicine, and it is still widely recommended for mental and physical well-being. RJ consists of a unique and complex mixture of multiple constituents in different concentrations, and some of its biological activities are directly associated with specific components not found elsewhere in nature, such as (E)-10-hydroxy-2-decenoic acid (10-HDA) and its precursor 10-hydroxydecanoic acid (10-HDAA), two medium-chain fatty acids. Together, 10-HAD and 10-HDAA represent the major constituents of the total lipid fraction in RJ, but despite their structural similarity, the former has been extensively investigated over the years, while the latter has been only marginally reported. This review focuses on the promising effects of 10-HDAA that have emerged in a series of recent in vitro, in vivo, and docking simulation studies. Important bioactivities were observed for 10-HDAA, tested both as an individual compound, especially for immunoregulatory, estrogenic, and anti-inflammatory activities, and in synergic combination with other molecules. Specific anti-infective effects against endemic diseases, as well as the structural modification to synthesize biocompatible and biodegradable 10-HDAA-based amphiphiles, are also reported.

Investigation of In Vitro and In Silico Anti-Inflammatory Potential of Carthamus caeruleus L. Root Juice

2025-06-21

Idir Moualek , Hamdi Bendif , Ali Dekir +5 more | International Journal of Molecular Sciences

This study aimed to evaluate the anti-inflammatory properties of Carthamus caeruleus L. root juice (CRJ), which is used in the traditional medicine of Algeria. The product was characterized by colorimetric … This study aimed to evaluate the anti-inflammatory properties of Carthamus caeruleus L. root juice (CRJ), which is used in the traditional medicine of Algeria. The product was characterized by colorimetric assays (total polyphenols, flavonoids, and tannins) and by RP-HPLC-DAD analysis. Experiments were conducted in vitro to assess the ability of CRJ to stabilize human erythrocyte membranes under various stress conditions and inhibit albumin denaturation, a process linked to inflammation. An in silico study was also performed to investigate the inhibitory effects on cyclooxygenase-2 (COX-2) and assess the phenolic constituents with the highest activity. Moderate levels of polyphenols, flavonoids, and tannins were assessed; among these, 22 compounds were identified via chromatographic analysis. While present at low concentrations, some of these compounds, including myricetin, luteolin, and quercetin, are known to exhibit bioactivity at micromolar levels. CRJ provided erythrocyte membranes with notable protection against disruption caused by hypotonic NaCl solutions (protection levels of 90.51%, 87.46%, and 76.87% at NaCl concentrations of 0.7%, 0.5%, and 0.3%, respectively), heat stress (81.54%), and oxidative damage from HClO (75.43%). Additionally, a protection of 61.5% was observed against albumin denaturation. Docking analysis indicated favorable COX-2 binding for myricetin, luteolin, and quercetin. In conclusion, the root juice derived from C. caeruleus demonstrated potential anti-inflammatory activity in vitro and in silico. However, further studies, including in vivo investigations, are necessary to confirm efficacy and fully elucidate the mechanisms of action.

Effect of Ascorbic Acid Addition on the Phenolic Compounds Content in Homogenates from Aerial Parts of Spearmint, Fennel, and Thyme

2025-06-21

Jan Třı́ska , Naděžda Vrchotová , Jan Strohalm +5 more | Foods

The paper deals with the investigation of the ascorbic acid influence on the analytical results of polyphenol content in the samples of the spearmint, fennel, and thyme homogenates. The homogenates … The paper deals with the investigation of the ascorbic acid influence on the analytical results of polyphenol content in the samples of the spearmint, fennel, and thyme homogenates. The homogenates without and with addition of ascorbic acid and water were prepared and stabilized by high-pressure treatment. Their analysis was accomplished by high-performance liquid chromatography (HPLC) with DAD detection and by combination of liquid chromatography with mass spectrometry (LC-MS). Volatile terpenes were analyzed in all homogenates by combination of gas chromatography with mass spectrometry technique (GC-MS). The content of polyphenols of acidic nature, e.g., rosmarinic acid, revealed the highest difference between analytical results of the samples with and without ascorbic acid. Finally, prepared herb homogenates are suitable food supplements, which will find increasing application in various food preparations.

Oracure: Efficacy of Honey and Split Gill Mushroom (Schizophyllum Commune) Extract in Reducing Staphylococcus Aureus Growth

2025-06-21

Princess Dianne P Abogar , Iya S Banaag , Divya P.S. +4 more | International journal of research and scientific innovation

Oral health has long been recognized as an essential element of general well-being, but millions of individuals still suffer from oral health annually. These conditions can result from poor hygiene … Oral health has long been recognized as an essential element of general well-being, but millions of individuals still suffer from oral health annually. These conditions can result from poor hygiene and cause dental-related problems. The rise of antibiotic-resistant bacteria, such as S. aureus, calls for alternative antimicrobial solutions. Honey and split gill mushroom has been traditionally used not only as a food, but also with therapeutic purposes, especially for the topical treatment of wounds. This study investigated the antibacterial potential of honey and split gill mushroom extracts against S. aureus growth using an experimental laboratory design. Extracts were extracted and analyzed using the Kirby-Bauer disk diffusion method against S. aureus strains, with statistical methods used to identify significant differences. Results showed that 100% honey extract exhibited a mean inhibition zone of 37.67 mm, classifying it as susceptible. In contrast, 100% split gill mushroom extract and the combination of honey and mushroom extract showed no inhibition, classifying them as resistant. The split gill mushroom showed no antibacterial activity in this study, and combining it with honey did not enhance effectiveness. These findings suggest that honey is a promising natural antimicrobial agent, while the mushroom extract was ineffective.

Cytotoxic and Metastatic Effects of Anatolian Propolis and Chemotherapeutic Agents (DOX, TAM, CLB) in 2D and 3D Breast Cancer Models

2025-06-20

Habibe Sema Arslan , Serap Yalçın Azarkan , Gamze Turna Saltoğlu | Journal of Apitherapy and Nature

This study aims to comparatively investigate the cytotoxic and metastatic effects of Anatolian propolis and chemotherapeutic agents (Doxorubicin- DOX, Tamoxifen- TAM, Chlorambucil- CLB) on two-dimensional (2D) and three-dimensional (3D) breast … This study aims to comparatively investigate the cytotoxic and metastatic effects of Anatolian propolis and chemotherapeutic agents (Doxorubicin- DOX, Tamoxifen- TAM, Chlorambucil- CLB) on two-dimensional (2D) and three-dimensional (3D) breast cancer cell cultures. The triple-negative MDA-MB-231 breast cancer cell line was cultured in 2D and 3D models. Anatolian propolis was prepared using ethanol extraction and applied to the cells alone or in combination with chemotherapeutic agents (DOX, TAM, CLB) at their respective IC50 doses. Cytotoxicity was assessed using the XTT assay, while cell migration and invasion were evaluated using wound healing and invasion assays. Propolis, when combined with chemotherapeutic agents, significantly inhibited cell proliferation and migration (p

Propolis-Loaded Liposomes (ProLip): A Nanoformulated Immunomodulator Targeting Breast Cancer via Macrophage Activation

2025-06-20

Hamidah Mohd Zain , Musthahimah Muhamad , Nik Nur Syazni Nik Mohamed Kamal | Research Square (Research Square)

<title>Abstract</title> Propolis, a natural remedy derived from bee by-products, is known for its immunomodulatory and anticancer properties. However, its clinical application is hindered by poor solubility and bioavailability. This study … <title>Abstract</title> Propolis, a natural remedy derived from bee by-products, is known for its immunomodulatory and anticancer properties. However, its clinical application is hindered by poor solubility and bioavailability. This study formulated a propolis-loaded liposome (ProLip) using the thin-film hydration technique (soy phospholipid-to-cholesterol ratio 6:1) to enhance its therapeutic effect. Encapsulation reduced the particle size of propolis from 402.77 ± 7.53 nm to 249.67 ± 5.79 nm and enhanced physicochemical properties, including a low polydispersity index (0.098 ± 0.02), highly negative zeta potential (-50.80 ± 0.10 mV), and improved solubility (water contact angle of 50.247°). FTIR analysis confirmed intermolecular interactions between phenolic groups in propolis and phospholipid carbonyl groups, while electron microscopy and surface morphology analysis revealed uniform structure and phagosomal localization in macrophages. Functionally, ProLip enhanced the secretion of anti-inflammatory cytokines IL-10 (49.429 ± 0.38 pg/mL) and IL-6 (40.488 ± 0.10 pg/mL), while suppressing pro-inflammatory mediators TNF-α and IL-1β by more than 80% compared to the LPS-treated group, highlighting ProLip as a potential immunoregulatory agent. Electron microscopy confirmed phagosomal localization of ProLip and reduced macrophage morphological damage compared to unencapsulated propolis, validating targeted delivery and protection capacity. Additionally, conditioned media from ProLip-treated macrophages significantly induced apoptosis (>50%) and inhibited migration and invasion in MCF-7 breast cancer cells, supporting immune-mediated anticancer effects. These findings highlight ProLip’s potential as a nanocarrier to enhance the bioavailability, cellular targeting, and therapeutic efficacy of stingless bee propolis in cancer immunotherapy.

Evaluation of the Antioxidant Activity of Honey

2025-06-20

Hiroko Seki | Journal of Food Engineering and Technology

Antioxidant activity of honey highly correlates with its total phenol content. The antioxidant capacity is attributable to various components, and there are several measurement methods for evaluating all of these … Antioxidant activity of honey highly correlates with its total phenol content. The antioxidant capacity is attributable to various components, and there are several measurement methods for evaluating all of these components; hence, integration of measurement methods is important. Therefore, the aim of this study was to evaluate the antioxidant activity of honey by examining correlations among the total polyphenol content, color values, melanoidin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, and iron-reducing capacity. Fourteen honey samples were collected from different nectar sources. Total polyphenol content was measured using the Folin–Ciocalteu method. Color values and melanoidin content were measured using the absorbance values of samples. The antioxidant capacity was evaluated by measuring DPPH radical-scavenging activity and iron-reducing ability using the ferric-reducing antioxidant power method. Substantial correlations were observed between the total polyphenol content and iron-reducing capacity, total polyphenol content and color value, color value and iron-reducing capacity, melanoidin content and total polyphenol content, melanoidin content and iron-reducing capacity, and melanoidin content and color value. Among all honey samples, buckwheat honey had the highest values for all parameters. These findings indicate that the antioxidant effect of honey should be evaluated based on not only its total polyphenol content and DPPH radical-scavenging activity but also its iron-reducing capacity, color value, and melanoidin content.

Antiviral Activity of Brazilian Propolis from Stingless Bees Against Rotavirus

2025-06-19

Guadalupe González-Ochoa , Ana Paola Balderrama-Carmona , Jesús Antonio Erro-Carvajal +3 more | Microorganisms

Group A rotavirus (RV) causes gastrointestinal disease in infants worldwide, and there is currently no specific treatment to eliminate the virus. Due to its chemical properties, propolis is a promising … Group A rotavirus (RV) causes gastrointestinal disease in infants worldwide, and there is currently no specific treatment to eliminate the virus. Due to its chemical properties, propolis is a promising compound for improving gastrointestinal infections. This study aimed to evaluate the action of stingless bee propolis against RV. The method involved determining the concentrations of the extracts that do not exhibit cytotoxicity in colon adenocarcinoma cells using the MTT assay and measuring the reduction in infectivity through a focus forming assay. The results showed that stingless bee propolis was non-cytotoxic up to 200 µg/mL. The reduction in RV infectivity exceeded 99% when using propolis from Plebeia droryana and Melipona quadrifasciata. Brazilian stingless bee propolis, whose active components are known for their activity against various viruses, was experimentally tested and demonstrated effective antiviral activity against RV, supporting its potential application as an antiviral agent.

Geopropolis from Melipona fasciculata Smith Accelerates Wound Healing in Diabetic Mice

2025-06-19

Aramys Silva Reis , Gabriela Corrêa Souza , Guilherme Martins Gomes Fontoura +7 more | Metabolites

Diabetic foot ulcers present a significant clinical challenge because of their high prevalence and severe complications. The need for innovative and accessible treatment options is critical. Owing to their medicinal … Diabetic foot ulcers present a significant clinical challenge because of their high prevalence and severe complications. The need for innovative and accessible treatment options is critical. Owing to their medicinal properties, natural products, such as geopropolis, hold promise. However, the wound healing potential of the geopropolis of Melipona fasciculata, particularly in accelerating the healing of diabetic ulcers, remains unexplored. In this study, we evaluated the ability of the geopropolis of M. fasciculata to promote wound healing in diabetic mice. Geopropolis was collected, prepared as a hydroalcoholic extract, and formulated into a topical cream. Non-obese diabetic (NOD) mice with induced chronic wounds were treated with this cream daily, and wound healing was assessed through macroscopic measurements, histological analysis, cytokine quantification, and in silico molecular docking studies. The results demonstrated that, compared with the control treatment, the geopropolis cream accelerated wound closure at all the analyzed time points (days 3, 7, and 14), reduced inflammatory infiltrates, and enhanced fibroblast proliferation and collagen deposition. These alterations were particularly pronounced in the final phase of healing, indicating an improvement in wound repair processes. These effects occurred without altering systemic cytokine levels, suggesting a localized treatment action. These results may be partially associated with the theoretical ability of beta-amyrin and cycloartenol to interact with human myeloperoxidase (MPO), as suggested by in silico docking analysis. Overall, the findings indicate that geopropolis cream could represent a viable alternative for managing diabetic ulcers, providing an effective means to enhance wound healing while remaining accessible to low-income populations.

UHPLC-ToF-MS approach for the analysis of antibiotic residues in Algerian honey: a preliminary assessment

2025-06-19

Moumeni Lynda , Ouchemoukh Salim , Leite Marta +7 more | Food Control

Propolis as a functional plant-derived food: antioxidant and anti-cancer properties from Şırnak and Hakkari Regions

2025-06-19

Mehmet Fidan , Behçet İnal , Onur Tokgün +3 more | European Food Research and Technology

Abstract This study evaluates the chemical composition, antioxidant activities, and anti-cancer potential of propolis from 12 key beekeeping regions in Şırnak and Hakkari. Significant variations in phytochemical compounds, such as … Abstract This study evaluates the chemical composition, antioxidant activities, and anti-cancer potential of propolis from 12 key beekeeping regions in Şırnak and Hakkari. Significant variations in phytochemical compounds, such as Chrysin, Caffeic Acid, Kaempferol, Rosmarinic acid, Quercetin-3-D-xyloside, and Quercetin, were identified. These compounds are known for their antioxidant and potential anti-cancer properties. Propolis extracts were obtained using Soxhlet extraction with n-hexane and ethanol, followed by GC–MS and LC–MS/MS analyses to identify volatile and phytochemical compounds. Antioxidant activity was assessed using DPPH and iron chelation assays. The anti-cancer potential of propolis was assessed against lung and breast cancer cell lines by evaluating cell proliferation and determining IC 50 values. The findings offer insights into the bioactive properties of the propolis from Şırnak for potential therapeutic applications. Preliminary cytotoxicity tests identified samples 3, 5, 6, 7, 9, and 10 as particularly effective. These samples were tested on lung (HUVEC, A549, H1975) and breast cancer cell lines (MDA-MB-231, MCF-7, MCF-10A) at various concentrations, with IC 50 values determined using the MTT assay. Propolis samples numbered 5, 7 and 9 showed significant activity in inhibiting cell proliferation and migration in breast cancer cell lines, while samples numbered 5, 7 and 10 were more effective in lung cancer cell lines. These findings underscore the potent antioxidant and anti-cancer properties of propolis, highlighting the significant influence of chemical composition and geographical origin. Further investigations, particularly in vivo and clinical studies, are essential to elucidate its therapeutic potential and underlying anti-cancer mechanisms.

A pH-Responsive strategy for rapid enrichment and purification of tropane alkaloids in honey based on zeolitic imidazolate Framework-8: Cost-effective and environmentally friendly

2025-06-19

Zile Wang , Yuliang Xu , Shuai Zhang +6 more | Microchemical Journal

Raman Spectroscopic Biodosimetry Using Protein in Murine Hair

2025-06-19

Spencer A. Witte , Monika Poonia , Martin J. Woodward +4 more | Radiation Research

Hair is an attractive sample for determining exposure to ionizing radiation due to its non-invasive nature. A biological tissue comprised mainly of keratin protein, hair is susceptible to oxidative or … Hair is an attractive sample for determining exposure to ionizing radiation due to its non-invasive nature. A biological tissue comprised mainly of keratin protein, hair is susceptible to oxidative or reductive stress by direct or indirect damage mechanisms. In this report, changes observed in the Raman spectra associated with hair protein from ionizing radiation were assessed for biodosimetry. Raman spectra were obtained from the hairs of a mixed sex cohort of irradiated C57BL/6 mice (N = 32 total) with doses of gamma rays ranging from 0–4 Gy. Radiation-dependent changes in the Raman spectra of the hairs provided molecular-specific signals that can inform about the damage mechanism. Partial least-squares discriminant analysis (PLS-DA) models incorporating automated variable selection for each sex showed classification of controls or exposed at 80% accuracy based on cross-validation. Models show only slight differences in performance based on the mouse’s sex from which the sample originated. This slight difference is consistent with PLS-DA models that show marginal cross-validation sensitivity (∼60%) in predicting the sex of the mouse from the Raman hair spectrum. Utilizing PLS regression, a dose-response model including both sexes showed root-mean-squared error (RMSE) ±1 Gy. The ability to determine dose or exposure from plucked hair with Raman spectroscopy would provide a needed tool for rapid medical triage after unexpected exposure.

Human health risk assessment of mercury in Apis mellifera honey samples from the Atlantic Forest in Bahia, Brazil

2025-06-18

Mário Marques da Silva , Jaílson Santos de Novaıs , Caio S.A. Felix +3 more | Journal of Food Composition and Analysis

Effects of a Stingless Bee Honey Mouth Rinse on Dental Plaque Accumulation: A Randomised Clinical Trial

2025-06-18

Misya Humaira Reduan , Nor Suhaira Zulkaflee , Muhammad Annurdin Sabarudin +3 more | Cureus

The effects of honey processing on nanoparticles in Astragali Radix decoction: Self-assembly, bioavailability, and bioactivity

2025-06-18

Weiye Zhang , Jinjing Song , Xiaojuan Su +5 more | Food Research International

Cosmeceutical and Wound-Healing Activities of Green Hydroxypropyl-β-Cyclodextrin-Glycerol-Based Satureja montana Extracts

2025-06-18

Lejsa Jakupović , Jakub W. Strawa , Laura Nižić Nodilo +6 more | Molecules

Satureja montana L. (winter savory, family Lamiaceae) is an aromatic herb that is widespread throughout the Mediterranean region. In a prior study, the optimization of the green hydroxypropyl-β-cyclodextrin (HP-β-CD)-glycerol-assisted extraction … Satureja montana L. (winter savory, family Lamiaceae) is an aromatic herb that is widespread throughout the Mediterranean region. In a prior study, the optimization of the green hydroxypropyl-β-cyclodextrin (HP-β-CD)-glycerol-assisted extraction procedure of S. montana was performed. As a result, four extracts abundant in total phenols (OPT-TP), total phenolic acids including rosmarinic acid (OPT-TPA-RA), total flavonoids (OPT-TF), and luteolin derivatives (OPT-LG) showing anti-elastase and anti-hyaluronidase properties, were prepared. Subsequently, we further explored the phytochemical, dermatological, and cosmeceutical potentials of these extracts, evaluating their antioxidant, anti-inflammatory, anti-tyrosinase, and anti-ultraviolet (UV) absorption activities. Furthermore, the biocompatibility of the extracts and their wound-healing properties were assessed using HaCaT cells. The results indicate that the extracts exhibited excellent antioxidant and cosmeceutical activities, which surpassed the activities of the employed standards in several assays (DPPH antiradical activity, β-carotene-linoleic acid, anti-lipoxygenase, anti-heat-induced ovalbumin coagulation, and UV absorbance assays). Furthermore, the extracts preserved more than 80% of the HaCaT cell viability at concentrations up to 62.5 µL extract/mL and also enhanced wound healing in the in vitro scratch wound-healing model. For example, the application of OPT-TP and OPT-TF led to 48.6% ± 3.3% and 48.6% ± 5.4% wound closure, respectively, after 48 h, compared to 34.8% ± 2.3% in the control group. The extracts exhibited excellent bioactivities, making them promising candidates for the development of cosmeceutical products, while their high biocompatibility indicates that they are suitable for direct application in cosmetics without prior solvent removal.

Chống biến màu và oxy hóa lipid trong cơ thịt cá ngừ vây vàng đông lạnh bằng nano chitosan-ergothioneine

2025-06-18

Bảo Huỳnh Nguyễn Duy , Nguyễn Trọng Bách , Nguyễn Hồng Ngân +4 more | Tạp chí Khoa học - Công nghệ Thủy Sản Trường Đại học Nha Trang

Nghiên cứu này nhằm đánh giá hiệu quả chống biến màu và chống oxy hóa lipid của nano chitosan tải ergothioneine (ECNP) đối với cơ thịt cá ngừ vây vàng … Nghiên cứu này nhằm đánh giá hiệu quả chống biến màu và chống oxy hóa lipid của nano chitosan tải ergothioneine (ECNP) đối với cơ thịt cá ngừ vây vàng (Thunnus albacares) bảo quản đông ở -20 ± 2°C. Hiệu quả được đánh giá thông qua các chỉ tiêu, bao gồm chỉ số màu đỏ (RI), hàm lượng metmyoglobin (metMb), lipid hydroperoxide (HPO) và các chất phản ứng với acid thiobarbituric (TBARS). Kết quả cho thấy ECNP có khả năng ức chế hiệu quả quá trình biến màu và oxy hóa lipid trong cơ thịt cá ngừ vây vàng bảo quản đông, với mức độ ức chế phụ thuộc vào liều lượng ECNP sử dụng. Sau 6 tháng bảo quản, mẫu cơ thịt cá ngừ vây vàng xử lý ECNP ở liều lượng 200 mg/kg duy trì hàm lượng metMb dưới 26%, RI lớn hơn 1 và giá trị TBARS thấp hơn 200 nmol MDA/g. Những phát hiện này chỉ ra rằng ECNP là một chất chống oxy hóa tự nhiên tiềm năng, có thể ứng dụng để hạn chế sự biến màu và quá trình oxy hóa lipid trong cơ thịt cá ngừ vây vàng đông lạnh. Từ khóa: bảo quản thủy sản, chống oxy hóa, metmyoglobin, sashimi, Thunnus albacares Abtract This study aimed to evaluate the anti-discoloration and lipid oxidation inhibitory effects of ergothioneine-loaded chitosan nanoparticles (ECNP) on the muscle of frozen yellowfin tuna (Thunnus albacares) stored at -20 ± 2°C. These effects were assessed based on key indicators, including redness index (RI), metmyoglobin (metMb) concentration, and the contents of lipid hydroperoxide (HPO) and thiobarbituric acid reactive substances (TBARS). The results demonstrated that ECNP effectively inhibited discoloration and lipid oxidation in frozen yellowfin tuna muscle, with the degree of inhibition depending on the ECNP dosage. After 6 months of storage, yellowfin tuna muscle treated with ECNP at 200 mg/kg maintained metMb concentration below 26%, RI greater than 1, and TBARS content lower than 200 nmol MDA/g. These findings suggest that ECNP is a promising natural antioxidant that can be applied to prevent discoloration and lipid oxidation in frozen yellowfin tuna muscle. Keywords: seafood preservation, antioxidant, metmyoglobin, sashimi, Thunnus albacares

A liquid chromatographic time-of-flight tandem mass spectrometric method for the comparative study of propolis aqueous and cyclodextrin extracts employing target and suspect screening

2025-06-18

Natasa P. Kalogiouri , Stamatia Christaki , Anastasia Loukri +2 more | Journal of Food Composition and Analysis

Herbal Medicine for Treating Herpes Labialis: A Systematic Review

2025-06-17

Melanie Anheyer , Holger Cramer , Thomas Ostermann +2 more | Journal of Integrative and Complementary Medicine

Introduction: Herpes labialis, commonly caused by herpes simplex virus type 1, affects millions globally and is traditionally managed with nucleoside antiviral drugs. However, increasing interest in complementary and integrative therapies … Introduction: Herpes labialis, commonly caused by herpes simplex virus type 1, affects millions globally and is traditionally managed with nucleoside antiviral drugs. However, increasing interest in complementary and integrative therapies has led to the exploration of topical herbal treatments as potential alternatives or adjuncts in managing this condition. Methods: A systematic review was conducted in accordance with Cochrane guidelines and Preferred Reporting Items for Systematic Reviews and Meta-Analyses recommendations, with prior registration. A comprehensive search of Medline/PubMed, Scopus, and the Cochrane Central Register of Controlled Trials was performed from inception to June 17, 2024. After screening 7,386 nonduplicate records and assessing 346 full-text articles, 7 randomized controlled trials (RCTs) encompassing 1,250 patients were included. Data were extracted regarding intervention types, outcomes, and adverse events, and the risk of bias was evaluated using the Cochrane Risk of Bias Tool. Results: The included studies evaluated topical formulations of lemon balm (Melissa officinalis L.), olive leaf extract (Olea europaea L.), propolis, and a combined sage-rhubarb cream (Salvia officinalis L., Rheum palmatum L., and Rheum officinale Baill.). Lemon balm preparations consistently reduced pain intensity and swelling, with some studies also reporting a significant decrease in lesion size compared with placebo or acyclovir. Olive leaf extract demonstrated a statistically significant faster improvement in symptoms and a shorter healing time compared with acyclovir. Compared with acyclovir, propolis formulations significantly shortened the median time to lesion encrustation and complete healing compared with acyclovir, and the combined sage-rhubarb cream showed a comparable efficacy to acyclovir in mean healing time. Overall, the risk of bias was judged to be low in two trials, while five trials raised some concerns. The total sample size across studies was small, potentially limiting the generalizability of the results. Across all studies, adverse events were minimal or absent. Discussion: The findings indicate that topical herbal therapies may provide effective and well-tolerated alternatives or adjuncts to conventional antiviral treatments for herpes labialis. However, considerable heterogeneity in intervention protocols and outcome measures, as well as the underrepresentation of pediatric populations, limit the generalizability of these findings. Conclusion: Topical herbal interventions, including lemon balm, olive leaf extract, propolis, and sage-rhubarb formulations, demonstrate potential benefits in managing herpes labialis with favorable safety profiles. Future well-designed, large-scale RCTs employing standardized methodologies are necessary to confirm these findings and to establish optimal treatment protocols for diverse patient populations (Inplasy protocol number: 202350038).

CU, FE, MN AND ZN CONTENTS IN BEE PROPOLIS FROM MALATYA, TURKEY AND HEALTH RISK ASSESSMENT

2025-06-17

Nagihan M. Karaaslan | İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi

In this study, copper (Cu), iron (Fe), manganese (Mn) and zinc (Zn) contents of propolis samples obtained from five different districts of Malatya, Türkiye were investigated. Element contents were determined … In this study, copper (Cu), iron (Fe), manganese (Mn) and zinc (Zn) contents of propolis samples obtained from five different districts of Malatya, Türkiye were investigated. Element contents were determined using flame atomic absorption spectrometry (FAAS). The concentration ranges of propolis samples taken from five different districts of Malatya province varied between 1.24 ± 0.06 mg/kg and 6.28 ± 0.02 mg/kg for Cu, 206.28 ± 21.50 mg/kg and 663.08 ± 55.24 mg/kg for Fe, 15.40 ± 0.17 mg/kg and 27.11 ± 1.83 for Mn, 39.36 ± 1.82 mg/kg and 52.57 ± 2.13 mg/kg for Zn. The results obtained for each district and element were evaluated in detail considering the estimated daily intake (EDI), recommended dietary allowance (RDA), provisional maximum tolerable daily intake (PMTDI) of these elements for adults. Moreover, hazard coefficient (HQ) was calculated for risk assessment and it was determined that HQ values for the studied elements in bee propolis from all districts were below 1.

Lignocellulose-rich walnut shell hydrochars for eco-friendly and biocompatible cosmetics: A sustainable strategy for product stability and microbial protection

2025-06-17

Erdal Yabalak , Hiba Alhousny | International Journal of Biological Macromolecules

Kinetics-thermodynamics of the antioxidant activity of propolis extracts in pork pâté

2025-06-17

José Dilson Francisco da Silva , Fernanda Cristina Breda , Lucila C. Ribeiro +6 more | Food Research International

Brazilian propolis: Chemical composition, regional variability, and bioactive potential

2025-06-16

Isabela Motta Felício , Andréa Cavalcanti , Kévin Baranger +4 more | Fitoterapia

Effect of simulated gastrointestinal digestion on phenolic compounds, anti-inflammatory, and antioxidant potential of Brazilian honeydew honey

2025-06-16

Siluana Katia Tischer Seraglio , Bibiana Silva , Eduarda Talita Bramorski Mohr +6 more | European Food Research and Technology

Optimization of a method for fermenting bee pollen: breaking down the grain wall and maximizing the release of phenolic compounds

2025-06-16

Gislaine Pucholobek , Yohandra Reyes Torres , Maidelen Del Carmen Lozano Guerrero +4 more | South African Journal of Botany

Ficha de Triportheus auritus

2025-06-16

André Luiz Henriques Esguícero , André Luiz Netto Ferreira , Bruno Francelino de Melo +7 more | Datasets - Sistema SALVE - ICMBio

Exploring Brazilian Green Propolis Phytochemicals in the Search for Potential Inhibitors of B-Raf600E Enzyme: A Theoretical Approach

2025-06-16

Garcia Ferreira de Souza , A. F. de Santana , Francisco Antunes +4 more | Pharmaceuticals

Background/Objectives: Melanoma is one of the most aggressive forms of skin cancer and is frequently associated with the B-Raf600E mutation, which constitutively activates the MAPK signaling pathway. Although selective inhibitors … Background/Objectives: Melanoma is one of the most aggressive forms of skin cancer and is frequently associated with the B-Raf600E mutation, which constitutively activates the MAPK signaling pathway. Although selective inhibitors such as Vemurafenib offer clinical benefits, their long-term efficacy is often hindered by resistance mechanisms and adverse effects. In this study, twelve phytochemicals from Brazilian green propolis were evaluated for their potential as selective B-Raf600E inhibitors using a computational approach. Methods: Physicochemical, ADME, and electronic properties were assessed, followed by molecular docking using the B-Raf600E crystal structure (PDB ID: 3OG7). Redocking validation and 500 ns molecular dynamics simulations were performed to investigate the stability of the ligand-protein complexes, and free energy calculations were then computed. Results: Among the tested compounds, Artepillin C exhibited the strongest binding affinity (-8.17 kcal/mol) in docking and maintained stable interactions with key catalytic residues throughout the simulation, also presenting free energy of binding ΔG of -20.77 kcal/mol. HOMO-LUMO and electrostatic potential analyses further supported its reactivity and selectivity. Notably, Artepillin C remained bound within the ATP-binding site, mimicking several critical interactions observed with Vemurafenib. Results: Among the tested compounds, Artepillin C exhibited the strongest binding affinity (-8.17 kcal/mol) and maintained stable interactions with key catalytic residues throughout the simulation. HOMO-LUMO and electrostatic potential analyses further supported its reactivity and selectivity. Notably, Artepillin C remained bound within the ATP-binding site, mimicking several critical interactions observed with Vemurafenib. Conclusions: These findings indicate that Artepillin C is a promising natural compound for further development as a selective B-Raf600E inhibitor and suggest its potential utility in melanoma treatment strategies. This study reinforces the value of natural products as scaffolds for targeted drug design and supports continued experimental validation.

The Effectiveness of a Topical Rosehip Oil Treatment on Facial Skin Characteristics: A Pilot Study on Wrinkles, UV Spots Reduction, Erythema Mitigation, and Age-Related Signs

2025-06-16

Diana Patricia Oargă , Mihaiela Cornea‐Cipcigan , Silvia Amalia Nemeș +1 more | Cosmetics

Skin aging is a complex process influenced by several factors, including UV exposure, environmental stressors, and lifestyle choices. The demand for effective, natural skincare products has driven research into plant-based … Skin aging is a complex process influenced by several factors, including UV exposure, environmental stressors, and lifestyle choices. The demand for effective, natural skincare products has driven research into plant-based oils rich in bioactive compounds. Rosehip oil has garnered attention for its high content of carotenoids, phenolics, and antioxidants, which are known for their anti-aging, photoprotective, and skin-rejuvenating properties. Despite the growing interest in rosehip oil, limited studies have investigated its efficacy on human skin using advanced imaging technologies. This study aims to fill this gap by evaluating the efficacy of cold-pressed Rosa canina seed oil on facial skin characteristics, specifically wrinkles, ultraviolet (UV) spot reduction, and erythema mitigation, using imaging technologies (the VISIA analysis system). Seed oil pressed from R. canina collected from the Băișoara area of Cluj County has been selected for this study due to its high carotenoid, phenolic, and antioxidant contents. The oil has also been analyzed for the content of individual carotenoids (i.e., lutein, lycopene, β Carotene, and zeaxanthin) using HPLC-DAD (High-Performance Liquid Chromatography—Diode Array Detector), along with lutein and zeaxanthin esters and diesters. After the preliminary screening of multiple Rosa species for carotenoid, phenolic, and antioxidant contents, the R. canina sample with the highest therapeutic potential was selected. A cohort of 27 volunteers (aged 30–65) underwent a five-week treatment protocol, wherein three drops of the selected rosehip oil were topically applied to the face daily. The VISIA imaging was conducted before and after the treatment to evaluate changes in skin parameters, including the wrinkle depth, UV-induced spots, porphyrins, and texture. Regarding the bioactivities, rosehip oil showed a significant total carotenoids content (28.398 μg/mL), with the highest levels in the case of the β-carotene (4.49 μg/mL), lutein (4.33 μg/mL), and zexanthin (10.88 μg/mL) contents. Results indicated a significant reduction in mean wrinkle scores across several age groups, with notable improvements in individuals with deeper baseline wrinkles. UV spots also showed visible declines, suggesting ideal photoprotective and anti-pigmentary effects attributable to the oil’s high vitamin A and carotenoid content. Porphyrin levels, often correlated with bacterial activity, decreased in most subjects, hinting at an additional antimicrobial or microbiome-modulatory property. However, skin responses varied, possibly due to individual differences in skin sensitivity, environmental factors, or compliance with sun protection. Overall, the topical application of R. canina oil appeared to improve the facial skin quality, reduce the appearance of age-related markers, and support skin health. These findings reinforce the potential use of rosehip oil in anti-aging skincare formulations. Further long-term, large-scale studies are warranted to refine dosing regimens, investigate mechanisms of action, and explore synergistic effects with other bioactive compounds.

Promising Dietary Supplements with Potential Senotherapeutic Effects: Aqueous Extracts from Enzymatically Hydrolysed Hemp Seed Cake Flour and Hemp Seed Protein Concentrate

2025-06-15

Anthea Miller , Inga Kwiecień , Marek Bednarski +7 more | Antioxidants

In the present study, the primary by-products of the hemp-seed oil process-hemp seed cake flour and hemp seed protein concentrate-underwent enzymatic hydrolysis using proteases and carbohydrases, either individually or in … In the present study, the primary by-products of the hemp-seed oil process-hemp seed cake flour and hemp seed protein concentrate-underwent enzymatic hydrolysis using proteases and carbohydrases, either individually or in combination. The effectiveness of these enzymatic treatments in releasing bioactive compounds was evaluated by assessing the antioxidant and anti-inflammatory properties of the aqueous extracts of both hydrolysed and untreated hemp by-products. The aim was to explore their potential senotherapeutic properties and promote their application as dietary supplements. Secondary metabolites such as flavonoids, phenolic acids, and catechins were analysed using high-performance liquid chromatography. Total phenolic, flavonoid, and protein contents were determined using spectrophotometric methods. Scavenging activity (2,2-Diphenyl-1-picrylhydrazyl scavenging assay (DPPH assay)), antioxidant power (Ferric reducing antioxidant power assay (FRAP assay)), and lipid peroxidation-reducing activity (thiobarbituric acid-reactive substance analysis) were assessed through in vitro assays. Possible anti-inflammatory effects were evaluated by assessing haemolysis inhibition. The impact of extracts on albumin glycation induced by exposure to fructose was also determined. To assess the toxicity of extracts, a zebrafish larvae model was employed. All extracts contained significant amounts of phenolic compounds, flavonoids, and proteins, and they exhibited notable activities in reducing lipid peroxidation and stabilising erythrocyte cell membranes. However, they did not significantly influence protein glycation (the glycation inhibition was only in the range of 15-40%). Our research demonstrates the substantial health-promoting potential, including senescence delay, of aqueous extracts from by-products of the hemp-seed oil process, which are available in large quantities and can serve as valuable supplements to support the health of animals, including humans, rather than being discarded as waste from oil production.

The Effect of Keratinase-psyllium Husk Formulated as Feed Additive for Trichobezoar Prevention in Feline

2025-06-14

D. Wang , Zhenlei Zhou | Indian Journal of Animal Research

Background: Trichobezoars represent a frequent digestive issue in cats, often resulting from excessive hair ingestion during grooming. This study evaluated the effects of keratinase-psyllium husk as a feed additive to … Background: Trichobezoars represent a frequent digestive issue in cats, often resulting from excessive hair ingestion during grooming. This study evaluated the effects of keratinase-psyllium husk as a feed additive to prevent trichobezoar formation. Methods: The trial included 20 healthy adult cats, randomly divided into three groups: KP-L (low-dose keratinase-psyllium husk), KP-H (high-dose keratinase-psyllium husk) and Control (standard diet without additives). Each group received their respective diet for pre-trial 3 days and a formal trial 14 days. Parameters analyzed included keratin degradation, fecal trichobezoar excretion and blood indicators to assess overall health status. Result: The findings demonstrated that cats in the KP-H group exhibited significantly higher keratin degradation and increased fecal trichobezoar excretion compared to the KP-L and Control groups. Both KP-L and KP-H groups showed improved hair elimination, though the effects were dose-dependent. No adverse effects on hematological or biochemical parameters were observed, indicating the safety of the additive. The study suggests that dietary supplementation with keratinase-psyllium husk effectively enhances hair degradation and trichobezoar excretion in cats, with higher doses showing greater efficacy. This additive presents a promising preventive approach for managing hairball formation in feline diets.

Siphonaxanthin inhibits the growth of breast cancer cell subtypes by modulating the expression of cellular proteins associated with antioxidant defence, cell survival and apoptosis signaling

2025-06-14

Yogendra Prasad Kavalappa , Nimish Mol Stephen , Kirubakaran Baskaran Jayalakshmi +4 more | Medical Oncology

Internet of Things-Based Automated Honey Harvesting: A Smartphone-Integrated Solution for Efficient Beekeeping

2025-06-14

Ari Devianto , Nur Alif Mardiyah , Muhammad Nasar | Journal of Artificial Intelligence and Engineering Applications (JAIEA)

Background: The low level of technological adoption in honeybee farming in Indonesia has directly impacted productivity, despite the country's vast potential as a leading global honey producer due to its … Background: The low level of technological adoption in honeybee farming in Indonesia has directly impacted productivity, despite the country's vast potential as a leading global honey producer due to its rich tropical rainforests. Since 2013, Indonesia has imported approximately 70% of its national honey demand. Therefore, technological innovation in beekeeping has become an urgent necessity. Objective: This study aims to design and develop an automated honey harvesting system based on the Internet of Things (IoT), integrated with a smartphone application. Research Method: This research is an experimental design and development study that integrates traditional beehives with mechanical and electronic technologies, including a DC motor, load cell sensor to measure honey weight, DHT11 sensor to monitor environmental temperature and humidity, Arduino Uno as the main controller, and a Wi-Fi module to interface with the smartphone application. The system is powered by solar energy to support operation in remote areas. Research Results: The result of this design is a prototype of an automatic honey harvesting device that enables real-time monitoring and control of the harvesting process via a smartphone application. Conclusion and Recommendations: This innovation is expected to improve the efficiency and productivity of honeybee farming, while also promoting digital transformation within Indonesia’s beekeeping sector. Keyword: Honey, Harvest, Automatic, Internet of Things

QUERCETINA: PESQUISAS, INVESTIGAÇÕES E APLICAÇÕES ENVOLVENDO DENTE-DE-LEÃO

2025-06-13

Valquíria Regina Bressan , Fabiele Bernardi , Keller Paulo Nicolini +1 more | Editora e-Publicar eBooks

Re-evaluating Honey Quality: Key Factors Influencing Its Purity and Excellence

2025-06-13

Melaku Tafese Awulachew | Deleted Journal

Honey quality is a growing global concern due to its importance in nutrition, health, and the food industry. The purpose of this research is to review the composition, physicochemical characteristics, … Honey quality is a growing global concern due to its importance in nutrition, health, and the food industry. The purpose of this research is to review the composition, physicochemical characteristics, and variables influencing honey quality. Honey, a natural sweet product produced by bees, contains over 180 essential components, including sugars, minerals, and nutrients that provide energy and enhance immunity. Its physicochemical properties, reducing sugars (>65%), moisture content (<25%), electrical conductivity (<0.8 mS/cm for blossom honey), acidity (<0.2%), and sucrose content (5%), influence its flavor, granulation, nutritional value, and storage. Other indicators, including diastase activity, proline, phenols, and pollen density, are vital for determining honey’s quality and origin. Honey is also rich in minerals, with potassium being the most common. The composition and quality of honey are significantly impacted by several factors, including the floral source, climate, beekeeping practices, collection methods, adulteration, processing, agrochemicals, storage conditions, and geographic origin. Standardized quality assessment criteria have been established to evaluate honey quality, ensuring it meets regulatory requirements and consumer expectations. As honey demand continues to rise, understanding these influencing factors is crucial for ensuring that the honey consumed meets high-quality standards and retains its beneficial properties. This review highlights the importance of assessing honey’s physicochemical properties and understanding the various factors that contribute to its overall quality, which is essential for both consumer safety and the honey industry’s growth.

Differentiation and classification of Australian single botanical origin honeys using spatially offset Raman spectroscopy

2025-06-13

Jeremy D. Landry , S. Jimkeli Singh , Samuel L. Stanley +4 more | Food Chemistry

Live Malassezia strains from the mucosa of patients with ulcerative colitis: pathogenic potential and environmental adaptations

2025-06-13

Yong‐Joon Cho , Seung Yong Shin , Juan Yang +7 more | mBio

ABSTRACT The human gut contains a diverse range of microorganisms, including bacteria, viruses, protozoa, and fungi. Although research has predominantly focused on bacterial populations, emerging evidence highlights the significant role … ABSTRACT The human gut contains a diverse range of microorganisms, including bacteria, viruses, protozoa, and fungi. Although research has predominantly focused on bacterial populations, emerging evidence highlights the significant role of the gut mycobiota, particularly in the context of inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn’s disease. This study investigates the intestinal mucosal mycobiota of UC patients, aiming to isolate and characterize live Malassezia fungal strains from the gut mucosa. Our analysis confirmed the presence of Malassezia in the intestinal mucosal layer of UC patients, with live Malassezia globosa strains being successfully isolated. As Malassezia is generally associated with the skin, the genomic and transcriptomic profiles and virulence of the M. globosa gut isolates were compared with those of the skin isolates. While both gut and skin isolates of M. globosa shared high genomic similarity, transcriptomic analysis revealed distinct responses to oxygen levels, suggesting niche-specific adaptation. Compared with the skin isolates, the gut isolates exhibited higher virulence in a dextran sulfate sodium-induced mouse colitis model, resulting in more severe disease, reduced survival rates, and elevated proinflammatory cytokine levels in the host. Our findings highlight the potential role of M. globosa in the pathogenesis of IBD and underscore the importance of niche-specific adaptations in fungal virulence. IMPORTANCE Malassezia fungi predominantly reside on human skin and are associated with several skin diseases, such as seborrheic dermatitis. They have also been implicated in various other diseases, including inflammatory bowel disease (IBD). While Malassezia DNA has been detected in many fungal microbiome studies using fecal samples, no previous research had isolated live Malassezia strains from the gut or confirmed that live Malassezia cells reside within the gut environment. In this study, we successfully isolated live Malassezia globosa strains from the gut mucosal surface of ulcerative colitis patients and compared them to M. globosa skin isolates. Our results revealed significant differences in pathogenicity between the gut and skin isolates and suggest the important role of M. globosa in the gut and its involvement in IBD.

Melasma como distúrbio multifatorial: evidências da influência da microbiota intestinal e da alimentação

2025-06-13

Samuel de Oliveira Caldeira , Maria Rosaria Romano , Mayara Almeida Varaschim +7 more | Brazilian Journal of Health Review

O melasma é uma dermatose crônica pigmentada multifatorial, comumente associada a fatores hormonais, exposição solar e predisposição genética. Recentemente, evidências apontam para a influência da microbiota intestinal e da alimentação … O melasma é uma dermatose crônica pigmentada multifatorial, comumente associada a fatores hormonais, exposição solar e predisposição genética. Recentemente, evidências apontam para a influência da microbiota intestinal e da alimentação na fisiopatologia dessa condição, por meio do eixo intestino-pele. Este estudo tem como objetivo revisar a literatura científica sobre a correlação entre o melasma, o estado da microbiota intestinal e padrões alimentares. Trata-se de uma revisão integrativa, realizada nas bases PubMed, Scopus e SciELO, com artigos publicados entre 2013 e 2024. Os dados analisados sugerem que desequilíbrios na microbiota (disbiose) podem amplificar processos inflamatórios e estressores oxidativos, influenciando a pigmentação cutânea. Além disso, padrões alimentares ricos em compostos antioxidantes, fibras e prebióticos demonstraram potencial modulador positivo sobre a microbiota e, indiretamente, sobre a expressão do melasma. Conclui-se que a modulação da microbiota por meio da dieta é uma abordagem promissora no manejo integrativo do melasma, abrindo caminho para estratégias terapêuticas inovadoras e individualizadas.

Microbial Contamination in Commercial Honey: Insights for Food Safety and Quality Control

2025-06-13

Felipe Bruxel , Ana Maria Geller , Andrei Giacchetto Felice +5 more | Microbiology Research

Honey is a sugar-rich product produced by Apis mellifera bees, with significant variability in properties due to the influence of geographic and climatic conditions and the predominant flora in the … Honey is a sugar-rich product produced by Apis mellifera bees, with significant variability in properties due to the influence of geographic and climatic conditions and the predominant flora in the production region. Economically, beekeeping is an activity that generates profit and fulfills environmental and social functions, reinforcing the pillars of sustainability. This study aimed to characterize samples of honey sold in southern Brazil, including physicochemical analyses, the detection of microbiological contaminants with potential impact on human health, and the detailed identification of bacterial composition through the Next-Generation Sequencing (NGS). The present study was divided into five main stages: (1) sample collection; (2) sample fractionation; (3) physicochemical analysis; (4) microbiological analysis; (5) 16S metataxonomy analysis. The physicochemical analyses agreed with the regulated values, indicating the good quality of the honey and the absence of adulteration. The microbiological analyses indicated the absence of Salmonella spp., in addition to a low count of total coliforms. The limits for molds and yeasts were exceeded in three samples, indicating non-compliance with current MERCOSUR legislation. Metabarcoding analysis identified a total of 15,736 OTUs divided into three different genera: Bacillus (41.54%), Lysinnibacillus, and Rossellomorea, all belonging to the Bacillaceae family. Some pathogenic species were identified, namely the Bacillus cereus group and Bacillus pumilus. Our results point to an increased need for surveillance, as honey contamination can lead to public health problems, requiring improvements in legislation and control parameters.

Therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss

2025-06-12

Abdellatif Boudra , Rachid Merati , Berrani Abdelkader +3 more | Revista Científica de la Facultad de Ciencias Veterinarias

This study investigates the therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss. It includes an in vitro phase to formulate an ethanolic extract of Algerian … This study investigates the therapeutic properties of Algerian propolis in skin wound healing with significant tissue loss. It includes an in vitro phase to formulate an ethanolic extract of Algerian propolis (EEPA) ointment and evaluate its antimicrobial activity, and an in vivo phase to compare its effects with silver sulfadiazine cream and a control group in rabbit wound healing. In vitro, two propolis samples (P1 and P2) were tested against Staphylococcus aureus, showing inhibition zones of 9.5 ± 1.04 mm (P1) and 11.8 ± 0.65 mm (P2). In vivo, the propolis–treated group (PG) achieved complete wound closure (16 cm²) within 16–30 days (d), compared to 24–36 d for the silver sulfadiazine group (SDG), while the control group (CG) did not achieve full closure after 36 d. Healing scores were highest in PG (2.27 ± 0.59 to 2.80 ± 0.46) and dressing evaluation scores were also superior ± 51). Faster fur regrowth was observed in PG, enhancing wound aesthetics. Postoperative hypothermia affected all groups, with CG experiencing the greatest temperature drop (>2°C) and higher mortality. In conclusion, Algerian propolis, particularly P2, exhibited superior activity due to its higher polyphenol content, demonstrating its potential as a natural antimicrobial agent shows strong potential in wound management by enhancing antimicrobial protection, accelerating healing, and improving wound aesthetics. Further studies are needed to optimize its application and elucidate its mechanisms of action.