Engineering › Electrical and Electronic Engineering

Electrochemical sensors and biosensors

Works Count: 86133

Wikipedia

Description

This cluster of papers focuses on the advances in electrochemical biosensor technology, with a particular emphasis on the use of graphene, carbon nanotubes, and nanomaterials for developing glucose sensors, enzymatic biofuel cells, and non-enzymatic sensors. The research covers various aspects of electrochemistry and highlights the applications of biosensors in diverse fields.

Keywords

Electrochemical Biosensors; Graphene; Glucose Sensors; Carbon Nanotubes; Enzymatic Biofuel Cells; Nanomaterials; Non-enzymatic Sensors; Electrochemistry; Biosensor Applications; Nanostructured Materials

[18] Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydroperoxides

1994-01-01

Simon P. Wolff

Quantitative Aspects of the Production of Superoxide Anion Radical by Milk Xanthine Oxidase

1970-08-01

Irwin Fridovich

At pH 7.0, in air, 20% of the total electron flux through xanthine oxidase can be accounted for in terms of the univalent reduction of oxygen. The fraction of the … At pH 7.0, in air, 20% of the total electron flux through xanthine oxidase can be accounted for in terms of the univalent reduction of oxygen. The fraction of the total flux of electrons which traversed the univalent pathway to oxygen was increased by raising the pH and by raising the oxygen tension. It was further shown that at any given pH and oxygen tension, the amount of univalently reduced oxygen, which was detectable in terms of the reduction of cytochrome c, rose as the turnover rate of the enzyme was decreased by decreasing the concentration of xanthine. This effect of xanthine was more pronounced at pH 7.0 than at pH 10.0. Another reflection of this same phenomenon was a difference in Km for xanthine measured in terms of urate production as compared to Km for xanthine measured in terms of cytochrome c reduction. Here too the differences were diminished as the pH and the oxygen tension were raised. The quantitative aspects of these phenomena are presented as well as an explanation which is consistent with all of the observations and which was, in fact, predictive of several of them.

The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters

1974-06-01

Robert Eisenthal , Athel Cornish‐Bowden

A new plot is described for analysing the results of kinetic experiments in which the Michaelis-Menten equation is obeyed. Observations are plotted as lines in parameter space, instead of points … A new plot is described for analysing the results of kinetic experiments in which the Michaelis-Menten equation is obeyed. Observations are plotted as lines in parameter space, instead of points in observation space. With appropriate modifications the plot is applicable to most problems of interest to the enzyme kineticist. It has the following advantages over traditional methods of plotting kinetic results: it is very simple to construct, because it is composed entirely of straight lines and requires no calculation or mathematical tables; the kinetic constants are read off the plot directly, again without calculation; it may be used during the course of an experiment to judge the success of the experiment, and to modify the experimental design; it provides clear and accurate information about the quality of the observations, and identifies aberrant observations; it provides a clear indication of the precision of the kinetic constants; constructed with care, it provides unbiased estimates of the kinetic constants, the same as those provided by a computer program; it may be used to simulate results for illustrative purposes very rapidly and simply.

View PDF Chat

USE OF GLUCOSE OXIDASE, PEROXIDASE, AND O-DIANISIDINE IN DETERMINATION OF BLOOD AND URINARY GLUCOSE

1957-08-01

A. St. G. Huggett , D.A. Nixon

Rotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layer

1980-07-01

Ralph A. Kamin , George S. Wilson

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layerRalph A. Kamin and George S. WilsonCite this: Anal. Chem. 1980, 52, 8, … ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layerRalph A. Kamin and George S. WilsonCite this: Anal. Chem. 1980, 52, 8, 1198–1205Publication Date (Print):July 1, 1980Publication History Published online1 May 2002Published inissue 1 July 1980https://pubs.acs.org/doi/10.1021/ac50058a010https://doi.org/10.1021/ac50058a010research-articleACS PublicationsRequest reuse permissionsArticle Views2551Altmetric-Citations1062LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

A new automated colorimetric method for measuring total oxidant status

2005-11-07

Özcan Erel

A spectrophotometric method for determination of catalase activity in small tissue samples

1988-10-01

Lars H. Johansson , Lisa Borg

Immobilization strategies to develop enzymatic biosensors

2011-09-18

Audrey Sassolas , Loı̈c J. Blum , Béatrice D. Leca‐Bouvier

Carbon‐Nanotube Based Electrochemical Biosensors: A Review

2004-11-19

Joseph Wang

Abstract This review addresses recent advances in carbon‐nanotubes (CNT) based electrochemical biosensors. The unique chemical and physical properties of CNT have paved the way to new and improved sensing devices, … Abstract This review addresses recent advances in carbon‐nanotubes (CNT) based electrochemical biosensors. The unique chemical and physical properties of CNT have paved the way to new and improved sensing devices, in general, and electrochemical biosensors, in particular. CNT‐based electrochemical transducers offer substantial improvements in the performance of amperometric enzyme electrodes, immunosensors and nucleic‐acid sensing devices. The greatly enhanced electrochemical reactivity of hydrogen peroxide and NADH at CNT‐modified electrodes makes these nanomaterials extremely attractive for numerous oxidase‐ and dehydrogenase‐based amperometric biosensors. Aligned CNT “forests” can act as molecular wires to allow efficient electron transfer between the underlying electrode and the redox centers of enzymes. Bioaffinity devices utilizing enzyme tags can greatly benefit from the enhanced response of the biocatalytic‐reaction product at the CNT transducer and from CNT amplification platforms carrying multiple tags. Common designs of CNT‐based biosensors are discussed, along with practical examples of such devices. The successful realization of CNT‐based biosensors requires proper control of their chemical and physical properties, as well as their functionalization and surface immobilization.

THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS

1949-05-01

George Scatchard

Glucose oxidase — An overview

2009-04-16

Sandip B. Bankar , Mahesh V. Bule , Rekha S. Singhal +1 more

3D Graphene–Cobalt Oxide Electrode for High-Performance Supercapacitor and Enzymeless Glucose Detection

2012-03-22

Xiaochen Dong , Hang Xu , Xuewan Wang +6 more

Using a simple hydrothermal procedure, cobalt oxide (Co(3)O(4)) nanowires were in situ synthesized on three-dimensional (3D) graphene foam grown by chemical vapor deposition. The structure and morphology of the resulting … Using a simple hydrothermal procedure, cobalt oxide (Co(3)O(4)) nanowires were in situ synthesized on three-dimensional (3D) graphene foam grown by chemical vapor deposition. The structure and morphology of the resulting 3D graphene/Co(3)O(4) composites were characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, and Raman spectroscopy. The 3D graphene/Co(3)O(4) composite was used as the monolithic free-standing electrode for supercapacitor application and for enzymeless electrochemical detection of glucose. We demonstrate that it is capable of delivering high specific capacitance of ∼1100 F g(-1) at a current density of 10 A g(-1) with excellent cycling stability, and it can detect glucose with a ultrahigh sensitivity of 3.39 mA mM(-1) cm(-2) and a remarkable lower detection limit of <25 nM (S/N = 8.5).

Determination of Glucose in Blood Using Glucose Oxidase with an Alternative Oxygen Acceptor

1969-01-01

P. Trinder

Electrochemical Glucose Sensors and Their Applications in Diabetes Management

2008-05-09

Adam Heller , Ben Feldman

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTElectrochemical Glucose Sensors and Their Applications in Diabetes ManagementAdam Heller, *† and Ben Feldman, ‡View Author Information Department of Chemical Engineering, University of Texas at Austin, Austin, … ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTElectrochemical Glucose Sensors and Their Applications in Diabetes ManagementAdam Heller, *† and Ben Feldman, ‡View Author Information Department of Chemical Engineering, University of Texas at Austin, Austin, Texas 78712, and Abbott Diabetes Care, 1360 South Loop Road, Alameda, California 94502* To whom correspondence should be addressed. E-mail: [email protected]; phone: 1-512-471-8874. †University of Texas at Austin.‡Abbott Diabetes Care.Cite this: Chem. Rev. 2008, 108, 7, 2482–2505Publication Date (Web):May 9, 2008Publication History Received17 September 2007Published online9 May 2008Published inissue 1 July 2008https://doi.org/10.1021/cr068069yCopyright © 2008 American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoiceArticle Views56196Altmetric-Citations1187LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1008 KB) Get e-AlertsSUBJECTS:Anatomy,Carbohydrates,Electrodes,Peptides and proteins,Redox reactions Get e-Alerts

Preparation, Structure, and Electrochemical Properties of Reduced Graphene Sheet Films

2009-06-22

Longhua Tang , Ying Wang , Yueming Li +3 more

Abstract This paper describes the preparation, characterization, and electrochemical properties of reduced graphene sheet films (rGSFs), investigating especially their electrochemical behavior for several redox systems and electrocatalytic properties towards oxygen … Abstract This paper describes the preparation, characterization, and electrochemical properties of reduced graphene sheet films (rGSFs), investigating especially their electrochemical behavior for several redox systems and electrocatalytic properties towards oxygen and some small molecules. The reduced graphene sheets (rGSs) are produced in high yield by a soft chemistry route involving graphite oxidation, ultrasonic exfoliation, and chemical reduction. Transmission electron microscopy (TEM), X‐ray diffraction (XRD), scanning electron microscopy (SEM), X‐ray photoelectron spectroscopy (XPS) and Raman spectroscopy clearly demonstrate that graphene was successfully synthesized and modified at the surface of a glassy carbon electrode. Several redox species, such as Ru(NH 3 ) 6 3+/2+ , Fe(CN) 6 3−/4− , Fe 3+/2+ and dopamine, are used to probe the electrochemical properties of these graphene films by using the cyclic voltammetry method. The rGSFs demonstrate fast electron‐transfer (ET) kinetics and possess excellent electrocatalytic activity toward oxygen reduction and certain biomolecules. In our opinion, this microstructural and electrochemical information can serve as an important benchmark for graphene‐based electrode performances.

Application of Nanoparticles in Electrochemical Sensors and Biosensors

2006-01-24

Xiliang Luo , Aoife Morrin , Anthony J. Killard +1 more

Abstract The unique chemical and physical properties of nanoparticles make them extremely suitable for designing new and improved sensing devices, especially electrochemical sensors and biosensors. Many kinds of nanoparticles, such … Abstract The unique chemical and physical properties of nanoparticles make them extremely suitable for designing new and improved sensing devices, especially electrochemical sensors and biosensors. Many kinds of nanoparticles, such as metal, oxide and semiconductor nanoparticles have been used for constructing electrochemical sensors and biosensors, and these nanoparticles play different roles in different sensing systems. The important functions provided by nanoparticles include the immobilization of biomolecules, the catalysis of electrochemical reactions, the enhancement of electron transfer between electrode surfaces and proteins, labeling of biomolecules and even acting as reactant. This minireview addresses recent advances in nanoparticle‐based electrochemical sensors and biosensors, and summarizes the main functions of nanoparticles in these sensor systems.

View PDF Chat

Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen

1969-03-01

P. Trinder

Manual and automated methods for the determination of blood glucose have been devised using an oxidase/peroxidase system, with dl adrenaline, a non-carcinogen, as oxygen acceptor. The manual technique employs a … Manual and automated methods for the determination of blood glucose have been devised using an oxidase/peroxidase system, with dl adrenaline, a non-carcinogen, as oxygen acceptor. The manual technique employs a stable single solution protein precipitant and the other reagents used are also stable. The automated methods are operated at 40/hr sample speed and washover between samples, over a very wide concentration range, is negligible.

View PDF Chat

Potential applications of enzymes immobilized on/in nano materials: A review

2011-09-18

Shakeel Ahmed Ansari , Qayyum Husain

A Stable Nonfluorescent Derivative of Resorufin for the Fluorometric Determination of Trace Hydrogen Peroxide: Applications in Detecting the Activity of Phagocyte NADPH Oxidase and Other Oxidases

1997-11-01

Mingjie Zhou , Zhenjun Diwu , Nataliya Panchuk-Voloshina +1 more

Glucose Biosensors: An Overview of Use in Clinical Practice

2010-05-04

Eun‐Hyung Yoo , Soo‐Youn Lee

Blood glucose monitoring has been established as a valuable tool in the management of diabetes. Since maintaining normal blood glucose levels is recommended, a series of suitable glucose biosensors have … Blood glucose monitoring has been established as a valuable tool in the management of diabetes. Since maintaining normal blood glucose levels is recommended, a series of suitable glucose biosensors have been developed. During the last 50 years, glucose biosensor technology including point-of-care devices, continuous glucose monitoring systems and noninvasive glucose monitoring systems has been significantly improved. However, there continues to be several challenges related to the achievement of accurate and reliable glucose monitoring. Further technical improvements in glucose biosensors, standardization of the analytical goals for their performance, and continuously assessing and training lay users are required. This article reviews the brief history, basic principles, analytical performance, and the present status of glucose biosensors in the clinical practice.

View PDF Chat

An improved colour reagent for the determination of blood glucose by the oxidase system

1972-01-01

Denise Barham , P. Trinder

A method involving the use of 4-aminophenazone as a colour coupler with sulphonated 2,4-dichlorophenol is described for determining the hydrogen peroxide produced from glucose with glucose oxidase.The sensitivity of the … A method involving the use of 4-aminophenazone as a colour coupler with sulphonated 2,4-dichlorophenol is described for determining the hydrogen peroxide produced from glucose with glucose oxidase.The sensitivity of the method is such that 20 µg of glucose in a final volume of 4 ml give an optical density of 0·61 at 515 nm with 10-mm cells, corresponding to a molecular absorption of 22 000.

Solubilization of Carbon Nanotubes by Nafion toward the Preparation of Amperometric Biosensors

2003-02-08

Joseph Wang , Mustafa Musameh , Yuehe Lin

The ability to solubilize single-wall and multiwall carbon nanotubes (CNT) in the presence of the perfluorinated polymer Nafion is described. Such use of Nafion as a solubilizing agent for CNT … The ability to solubilize single-wall and multiwall carbon nanotubes (CNT) in the presence of the perfluorinated polymer Nafion is described. Such use of Nafion as a solubilizing agent for CNT overcomes a major obstacle for creating CNT-based biosensing devices. Their association with Nafion does not impair the electrocatalytic properties of CNT. The resulting CNT/Nafion modified glassy-carbon electrodes exhibit a strong and stable electrocatalytic response toward hydrogen peroxide. The marked acceleration of the hydrogen peroxide redox process is very attractive for the operation of oxidase-based amperometric biosensors, as illustrated for the highly selective low-potential (-0.05 V vs Ag/AgCl) biosensing of glucose. These findings open the door for using CNT in a wide range of chemical sensors and nanoscale electronic devices.

Nitrogen-Doped Graphene and Its Application in Electrochemical Biosensing

2010-04-07

Ying Wang , Yuyan Shao , Dean W. Matson +2 more

Chemical doping with foreign atoms is an effective method to intrinsically modify the properties of host materials. Among them, nitrogen doping plays a critical role in regulating the electronic properties … Chemical doping with foreign atoms is an effective method to intrinsically modify the properties of host materials. Among them, nitrogen doping plays a critical role in regulating the electronic properties of carbon materials. Recently, graphene, as a true two-dimensional carbon material, has shown fascinating applications in bioelectronics and biosensors. In this paper, we report a facile strategy to prepare N-doped graphene by using nitrogen plasma treatment of graphene synthesized via a chemical method. Meanwhile, a possible schematic diagram has been proposed to detail the structure of N-doped graphene. By controlling the exposure time, the N percentage in host graphene can be regulated, ranging from 0.11 to 1.35%. Moreover, the as-prepared N-doped graphene has displayed high electrocatalytic activity for reduction of hydrogen peroxide and fast direct electron transfer kinetics for glucose oxidase. The N-doped graphene has further been used for glucose biosensing with concentrations as low as 0.01 mM in the presence of interferences.

Ferrocene-mediated enzyme electrode for amperometric determination of glucose

1984-04-01

Anthony E. G. Cass , G.B. Davis , Graeme D. Francis +6 more

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFerrocene-mediated enzyme electrode for amperometric determination of glucoseAnthony E. G. Cass, Graham. Davis, Graeme D. Francis, H. Allen O. Hill, William J. Aston, I. John. Higgins, Elliot … ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFerrocene-mediated enzyme electrode for amperometric determination of glucoseAnthony E. G. Cass, Graham. Davis, Graeme D. Francis, H. Allen O. Hill, William J. Aston, I. John. Higgins, Elliot V. Plotkin, Lesley D. L. Scott, and Anthony P. F. TurnerCite this: Anal. Chem. 1984, 56, 4, 667–671Publication Date (Print):April 1, 1984Publication History Published online1 May 2002Published inissue 1 April 1984https://pubs.acs.org/doi/10.1021/ac00268a018https://doi.org/10.1021/ac00268a018research-articleACS PublicationsRequest reuse permissionsArticle Views8874Altmetric-Citations1386LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

Electrochemical Glucose Biosensors

2007-12-23

Joseph Wang

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrochemical Glucose BiosensorsJoseph WangView Author Information Biodesign Institute, Center for Bioelectronics and Biosensors, Departments of Chemical Engineering and Chemistry and Biochemistry, Box 875801, Arizona State University, Tempe, … ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrochemical Glucose BiosensorsJoseph WangView Author Information Biodesign Institute, Center for Bioelectronics and Biosensors, Departments of Chemical Engineering and Chemistry and Biochemistry, Box 875801, Arizona State University, Tempe, Arizona 85287-5801 Cite this: Chem. Rev. 2008, 108, 2, 814–825Publication Date (Web):December 23, 2007Publication History Received29 March 2007Published online23 December 2007Published inissue 1 February 2008https://pubs.acs.org/doi/10.1021/cr068123ahttps://doi.org/10.1021/cr068123aresearch-articleACS PublicationsCopyright © 2008 American Chemical SocietyRequest reuse permissionsArticle Views43427Altmetric-Citations2850LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Anatomy,Carbohydrates,Electrodes,Peptides and proteins,Sensors Get e-Alerts

"Plugging into Enzymes": Nanowiring of Redox Enzymes by a Gold Nanoparticle

2003-03-20

Yi Xiao , Fernando Patolsky , Eugenii Katz +2 more

The reconstitution of an apo-flavoenzyme, apo-glucose oxidase, on a 1.4-nanometer gold nanocrystal functionalized with the cofactor flavin adenine dinucleotide and integrated into a conductive film yields a bioelectrocatalytic system with … The reconstitution of an apo-flavoenzyme, apo-glucose oxidase, on a 1.4-nanometer gold nanocrystal functionalized with the cofactor flavin adenine dinucleotide and integrated into a conductive film yields a bioelectrocatalytic system with exceptional electrical contact with the electrode support. The electron transfer turnover rate of the reconstituted bioelectrocatalyst is approximately 5000 per second, compared with the rate at which molecular oxygen, the natural cosubstrate of the enzyme, accepts electrons (approximately 700 per second). The gold nanoparticle acts as an electron relay or "electrical nanoplug" for the alignment of the enzyme on the conductive support and for the electrical wiring of its redox-active center.

Electrochemical Biosensing Platforms Using Platinum Nanoparticles and Carbon Nanotubes

2003-12-18

Sabahudin Hrapovic , Yali Liu , Keith B. Male +1 more

Platinum nanoparticles with a diameter of 2-3 nm were prepared and used in combination with single-wall carbon nanotubes (SWCNTs) for fabricating electrochemical sensors with remarkably improved sensitivity toward hydrogen peroxide. … Platinum nanoparticles with a diameter of 2-3 nm were prepared and used in combination with single-wall carbon nanotubes (SWCNTs) for fabricating electrochemical sensors with remarkably improved sensitivity toward hydrogen peroxide. Nafion, a perfluorosulfonated polymer, was used to solubilize SWCNTs and also displayed strong interactions with Pt nanoparticles to form a network that connected Pt nanoparticles to the electrode surface. TEM and AFM micrographs illustrated the deposition of Pt nanoparticles on carbon nanotubes whereas cyclic voltammetry confirmed an electrical contact through SWCNTs between Pt nanoparticles and the glassy carbon (GC) or carbon fiber backing. With glucose oxidase (GOx) as an enzyme model, we constructed a GC or carbon fiber microelectrode-based biosensor that responds even more sensitively to glucose than the GC/GOx electrode modified by Pt nanoparticles or CNTs alone. The response time and detection limit (S/N = 3) of this biosensor was determined to be 3 s and 0.5 microM, respectively.

Low-potential stable NADH detection at carbon-nanotube-modified glassy carbon electrodes

2002-10-01

Mustafa Musameh , Joseph Wang , Arben Merkoçi +1 more

Application of graphene-modified electrode for selective detection of dopamine

2009-02-22

Ying Wang , Yueming Li , Longhua Tang +2 more

Nanostructuring electrodes with carbon nanotubes: A review on electrochemistry and applications for sensing

2005-04-24

J. Justin Gooding

Nitric oxide release from a single cell measured in situ by a porphyrinic-based microsensor

1992-08-01

Tadeusz Maliñski , Ziad Taha

Direct Electrochemistry of Glucose Oxidase and Biosensing for Glucose Based on Graphene

2009-02-19

Changsheng Shan , Huafeng Yang , Jiangfeng Song +3 more

We first reported that polyvinylpyrrolidone-protected graphene was dispersed well in water and had good electrochemical reduction toward O2 and H2O2. With glucose oxidase (GOD) as an enzyme model, we constructed … We first reported that polyvinylpyrrolidone-protected graphene was dispersed well in water and had good electrochemical reduction toward O2 and H2O2. With glucose oxidase (GOD) as an enzyme model, we constructed a novel polyvinylpyrrolidone-protected graphene/polyethylenimine-functionalized ionic liquid/GOD electrochemical biosensor, which achieved the direct electron transfer of GOD, maintained its bioactivity and showed potential application for the fabrication of novel glucose biosensors with linear glucose response up to 14 mM.

Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader

1981-10-01

Edgar Pick , Diane Mizel

Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E

1999-05-01

Pilar Prieto , Manuel Pineda , Miguel Aguilar

View PDF Chat

Glucose oxidase: an ideal enzyme

1992-01-01

Robert Wilson , Anthony Turner

Application of chitin- and chitosan-based materials for enzyme immobilizations: a review

2004-06-22

Barbara Krajewska

The Enzyme Electrode

1967-06-01

Stuart J. Updike , G. P. Hicks

Graphene-based materials in electrochemistry

2010-01-01

Da Chen , Longhua Tang , Jinghong Li

Graphene, as the fundamental 2D carbon structure with exceptionally high crystal and electronic quality, has emerged as a rapidly rising star in the field of material science. Its sudden discovery … Graphene, as the fundamental 2D carbon structure with exceptionally high crystal and electronic quality, has emerged as a rapidly rising star in the field of material science. Its sudden discovery in 2004 led to an explosion of interest in the study of graphene with respect to its unique physical, chemical, and mechanical properties, opening up a new research area for materials science and condensed-matter physics, and aiming for wide-ranging and diversified technological applications. In this critical review, we will describe recent advances in the development of graphene-based materials from the standpoint of electrochemistry. To begin with, electron transfer properties of graphene will be discussed, involving its unusual electronic structure, extraordinary electronic properties and fascinating electron transport. The next major section deals with the exciting progress related to graphene-based materials in electrochemistry since 2004, including electrochemical sensing, electrochemiluminescence, electrocatalysis, electrochemical energy conversion and FET devices. Finally, prospects and further developments in this exciting field of graphene-based materials are also suggested (224 references).

Electrochemical non-enzymatic glucose sensors

2005-07-13

Se Jin Park , Hankil Boo , Taek Dong Chung

Graphene-based nanomaterials and their electrochemistry

2010-01-01

Martin Pumera

Graphene-based nanomaterials are in the forefront of chemical research. This tutorial review provides an introduction to their electrochemistry, its fundamentals and applications. Selected examples of applications in energy storage and … Graphene-based nanomaterials are in the forefront of chemical research. This tutorial review provides an introduction to their electrochemistry, its fundamentals and applications. Selected examples of applications in energy storage and sensing are presented. The synthetic methods for preparing graphenes as well as their materials chemistry are thoroughly discussed, as they have a profound influence on the electronic and electrochemical behavior of graphene-related nanomaterials. Inherent electrochemistry and spectroelectrochemistry of graphene nanomaterials is discussed thoroughly. Important application in sensing and energy storage areas are highlighted.

Graphene for electrochemical sensing and biosensing

2010-07-17

Martin Pumera , Adriano Ambrosi , Alessandra Bonanni +2 more

View PDF Chat

Graphene Based Electrochemical Sensors and Biosensors: A Review

2010-03-30

Yuyan Shao , Jun Wang , Hong Wu +3 more

Abstract Graphene, emerging as a true 2‐dimensional material, has received increasing attention due to its unique physicochemical properties (high surface area, excellent conductivity, high mechanical strength, and ease of functionalization … Abstract Graphene, emerging as a true 2‐dimensional material, has received increasing attention due to its unique physicochemical properties (high surface area, excellent conductivity, high mechanical strength, and ease of functionalization and mass production). This article selectively reviews recent advances in graphene‐based electrochemical sensors and biosensors. In particular, graphene for direct electrochemistry of enzyme, its electrocatalytic activity toward small biomolecules (hydrogen peroxide, NADH, dopamine, etc.), and graphene‐based enzyme biosensors have been summarized in more detail; Graphene‐based DNA sensing and environmental analysis have been discussed. Future perspectives in this rapidly developing field are also discussed.

View PDF Chat

Glucose Oxidase–graphene–chitosan modified electrode for direct electrochemistry and glucose sensing

2009-09-07

Xinhuang Kang , Jun Wang , Hong Wu +3 more

Enzymatic Biofuel Cells for Implantable and Microscale Devices

2004-09-21

Scott Calabrese Barton , Josh Gallaway , Plamen Atanassov

ADVERTISEMENT RETURN TO ISSUEPREVArticleEnzymatic Biofuel Cells for Implantable and Microscale DevicesScott Calabrese Barton, Josh Gallaway, and Plamen AtanassovView Author Information Department of Chemical Engineering, Columbia University, New York, New York … ADVERTISEMENT RETURN TO ISSUEPREVArticleEnzymatic Biofuel Cells for Implantable and Microscale DevicesScott Calabrese Barton, Josh Gallaway, and Plamen AtanassovView Author Information Department of Chemical Engineering, Columbia University, New York, New York 10027, and Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, New Mexico 87131 Cite this: Chem. Rev. 2004, 104, 10, 4867–4886Publication Date (Web):September 21, 2004Publication History Received26 July 2004Published online21 September 2004Published inissue 1 October 2004https://pubs.acs.org/doi/10.1021/cr020719khttps://doi.org/10.1021/cr020719kresearch-articleACS PublicationsCopyright © 2004 American Chemical SocietyRequest reuse permissionsArticle Views11434Altmetric-Citations1257LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biofuels,Electrodes,Fuel cells,Peptides and proteins,Redox reactions Get e-Alerts

The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay

1996-07-01

Iris F. F. Benzie , JJ Strain

Biosensors and their applications – A review

2016-01-06

Parikha Mehrotra

View PDF Chat

A graphene-based electrochemical device with thermoresponsive microneedles for diabetes monitoring and therapy

2016-03-18

Hyunjae Lee , Tae‐Kyu Choi , Young Bum Lee +7 more

Cyclodextrins and their applications in analytical chemistry

1992-09-01

Li Song , William C. Purdy

ADVERTISEMENT RETURN TO ISSUEPREVArticleCyclodextrins and their applications in analytical chemistrySong Li and William C. PurdyCite this: Chem. Rev. 1992, 92, 6, 1457–1470Publication Date (Print):September 1, 1992Publication History Published online1 May … ADVERTISEMENT RETURN TO ISSUEPREVArticleCyclodextrins and their applications in analytical chemistrySong Li and William C. PurdyCite this: Chem. Rev. 1992, 92, 6, 1457–1470Publication Date (Print):September 1, 1992Publication History Published online1 May 2002Published inissue 1 September 1992https://doi.org/10.1021/cr00014a009Request reuse permissionsArticle Views3817Altmetric-Citations854LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-Alertsclose Get e-Alerts

[7] Mitochondrial respiratory control and the polarographic measurement of ADP : O ratios

1967-01-01

Ronald W. Estabrook

Optimization of voltammetric parameters and sensitive determination of hazardous 2-nitrophenol in environmental media using a modified glassy carbon electrode based on 2-amino nicotinamide

2025-06-26

Zerrin Ansen , Tuğba Tabanlıgil Calam | Journal of Water Process Engineering

Development of Molecularly Imprinted Conducting Polymers for Stress Biomarker Cortisol Detection

2025-06-25

Wonhyeong Kim , Lang Zhou , Yoo Lim +2 more | ACS Applied Polymer Materials

Enhanced azo dye (Sudan G) decolorization and simultaneous electricity generation using a bacterial consortium in a dual-chamber microbial fuel cell

2025-06-25

Chinwendu Jude Echejiuba , Chimezie Jason Ogugbue , Ifeyinwa S. Obuekwe | Studia Universitatis Babeş-Bolyai Biologia

Azo dyes are prevalent anthropogenic compounds, making their enhanced treatment crucial in our color-saturated world. This study examined the ability of a microbial consortium, comprising Pseudomonas aeruginosa (MW584979), Enterobacter hormaechei … Azo dyes are prevalent anthropogenic compounds, making their enhanced treatment crucial in our color-saturated world. This study examined the ability of a microbial consortium, comprising Pseudomonas aeruginosa (MW584979), Enterobacter hormaechei (MW584986), Providencia stuartii (MW584987), Escherichia coli (MZ394117), and Pseudomonas xiamenensis (MW585052), to decolorize Sudan orange G in a microbial fuel cell (MFC) after determining the optimal conditions for dye decoorization using response surface methodology (RSM) and the One Factor at a Time (OFAT) method. Degradation products were analyzed using the gas chromatography-mass spectroscopy technique. The consortium achieved an 88% decolorization rate within 24 hours under the optimal conditions identified by the Central Composite Design (CCD) of RSM. These conditions, pH 7.0, temperature 35, salinity 5 g/L, and glucose concentration 10 g/L, when applied in the MFC, resulted in an enhanced decolorization rate of 92% and simultaneous electricity generation of 130 mV within 24 hours. GC-MS analysis confirmed the breakdown of the azo dye into simpler, less toxic compounds. Metabolites produced through RSM and MFC processes were identified and compared with controls using chromatography-mass spectrometry. Degradation metabolites obtained after treatment of the dye wastewater in the MFC include Cyclopentane and cyclopropylidene-2(1H)-naphthalenone which highlights the role of microbial enzymatic activity in converting complex azo dye structures into environmentally benign compounds. These results highlight the successful integration of RSM for process optimization and MFCs for enhanced biodegradation and renewable energy production. The scalability of this technique is promising, given the relatively simple and cost-effective setup of MFC systems. Moreover, the economic feasibility of large-scale deployment is enhanced by the dual benefits of wastewater treatment and renewable energy production, making it a sustainable solution for managing azo dye pollution. Article history: Received 7 August 2024; Revised 3 December 2024;Accepted 22 May 2025; Available online 25 June 2025

Controlled Release of Perillyl Alcohol via pH-Responsive Chitosan-Polypyrrole Nanocarriers

2025-06-25

Marcella Matos Cordeiro Borges , Stephanne Yonara Barbosa de Carvalho , Keyller Bastos Borges +1 more | ACS Omega

3D-Printed Boron-Nitrogen Doped Carbon Electrodes for Sustainable Wastewater Treatment via MPECVD

2025-06-24

Iwona Kaczmarzyk , Małgorzata Szopińska , Patryk Sokołowski +7 more | Nano-Micro Letters

This study proposes a novel and sustainable method for fabricating 3D-printed carbon-based electrodes for electrochemical wastewater treatment. We prepared B,N-doped carbon electrodes with hierarchical porosity and a significantly enhanced surface … This study proposes a novel and sustainable method for fabricating 3D-printed carbon-based electrodes for electrochemical wastewater treatment. We prepared B,N-doped carbon electrodes with hierarchical porosity and a significantly enhanced surface area-to-volume ratio (up to 180%) compared to non-optimized analogues using a synergistic combination of 3D printing, phase inversion, and microwave plasma-enhanced chemical vapor deposition. This process allows the metal-free growth of vertically aligned carbon nanostructures directly onto polymer-derived substrates, resulting in a 20-fold increase in the electrochemically active surface area. Computational fluid dynamics simulations were used to improve mass transport and reduce pressure drop. Electrochemical characterization demonstrated that the optimized electrodes performed significantly better, achieving 4.7-, 4-, and 6.5-fold increases in the degradation rates of atenolol, metoprolol, and propranolol, respectively, during electrochemical oxidation. These results highlight the efficacy of the integrated fabrication and simulation approach in producing high-performance electrodes for sustainable wastewater treatment applications.

Highly Specific and Fast-Responsive Semicarbazide-Sensitive Amine Oxidase Nanoprobe for the Serological Diagnosis of Parkinson’s Patients

2025-06-24

Yuankun Zhao , BH Liu , Hao Wang +7 more | Analytical Chemistry

Semicarbazide-sensitive amine oxidase (SSAO), a multifunctional enzyme associated with various diseases, including neurodegenerative disorders, has emerged as a potential biomarker for Parkinson's disease (PD). However, current SSAO detection methods, such … Semicarbazide-sensitive amine oxidase (SSAO), a multifunctional enzyme associated with various diseases, including neurodegenerative disorders, has emerged as a potential biomarker for Parkinson's disease (PD). However, current SSAO detection methods, such as immunoassays and mass spectrometry, are limited by their complexity, nonselectivity, high cost, and the use of hazardous radioactive materials. In this study, we developed a highly specific, sensitive, and cost-effective method for SSAO activity detection based on optical gold nanoparticles (AuNPs). By functionalizing AuNPs with rationally designed β-mercaptoethylamine (MEA), the nanoprobe AuNPs@MEA enabled SSAO-specific oxidation, leading to the formation of aldehyde groups and subsequently mediating AuNPs' aggregation via both Schiff base coupling and electrostatic interactions. This distinct change in the absorption spectrum allowed for quantitative detection of SSAO with a limit of detection (LOD) of 0.252 ng/mL. Such a nanoprobe was capable of sensitive SSAO detection in the in vitro PD model based on brain biochips, as well as enabling practical analysis of clinical human serum samples. Application of AuNPs@MEA to PD patient serum diagnosis revealed significantly elevated SSAO compared to healthy controls, indicating its high correlation with the pathogenesis of PD (n = 20). This nanoprobe also offered advantages for on-site serological diagnostic needs, such as minuscule sample volume (5 μL), fast response (<5 min), and high-throughput features, exhibiting potential for clinical translations.

Non-enzymatic electrochemical sensor for the reliable determination of catechol using RuS2/WO3/MWCNT ternary composite

2025-06-24

S. Sreelekshmi , Beena Saraswathyamma | Carbon letters

Conductance Sensitivity Evaluation of Trilayer Graphene Nanoribbon -Based Electrochemical Biosensor for Charged Lipid Bilayer Detection

2025-06-24

M. Rahmani , Zahra Rahmani , Mohammad Taghi Ahmadi | Journal of The Electrochemical Society

Abstract The performance of an electrolyte-gated field-effect transistor based on trilayer graphene nanoribbon (TGN) and adsorption effect on sensing parameters was investigated. In the proposed model, TGN carrier concentration as … Abstract The performance of an electrolyte-gated field-effect transistor based on trilayer graphene nanoribbon (TGN) and adsorption effect on sensing parameters was investigated. In the proposed model, TGN carrier concentration as a function of electric charge and thickness of the charged lipid bilayer (CLB) is considered and to obtain the characteristics including quantum capacitance, conductance and current-voltage characteristic, the suitable control parameters are defined. Moreover, TGN conductance as a electrical detection platform of each electric charge membrane together with membrane thickness is analytically investigated. The obtained results confirm that the minimum level of conductance of the biosensor is decreased and Vg-min is obviously left-shifted by increasing value of membrane thickness. In order to verify the performance of proposed biosensor, it is compared with recent theoretical and experimental reports of CLB biosensors. The analytical model of TGN-based electrochemical biosensor for CLB detection has not been performed until now, so computational investigation of the device performance and adsorption effect on the sensing parameters is one of the interesting steps in this study. As a result, the proposed TGN-based biosensor with significant conductance sensitivity and high current-voltage characteristic exposes relatively higher performance compared to those of CLB counterparts for analogous ambient conditions.

Melamine-Derived Molecularly Imprinted Polymer on a Low-Cost Pencil Graphite Electrode Electrochemical Sensor for Sensitive Thiamethoxam Detection

2025-06-24

Xiang Li , Jianshe Tang , Xinyu Wang +1 more | Journal of The Electrochemical Society

Abstract A novel electrochemical sensor was developed for the sensitive and selective detection of thiamethoxam, leveraging melamine’s multi-orientation polymerization via in-situ electropolymerization on a pencil graphite electrode. The molecularly imprinted … Abstract A novel electrochemical sensor was developed for the sensitive and selective detection of thiamethoxam, leveraging melamine’s multi-orientation polymerization via in-situ electropolymerization on a pencil graphite electrode. The molecularly imprinted polymer membrane was electrochemically deposited using cyclic voltammetry, with thiamethoxam as the template to create specific binding sites. After template removal, the molecularly imprinted polymer-modified pencil graphite electrode was characterized by scanning electron microscopy and cyclic voltammetry, confirming the successful formation of a porous imprinted layer with enhanced electron transfer resistance. The differential pulse voltammetry signal decline, attributed to the competitive binding of thiamethoxam at the MIP sites, showed a linear relationship with thiamethoxam concentrations over a wide range (100 nM - 200 μM), with a low limit of detection (100 nM). The sensor exhibited excellent selectivity toward thiamethoxam, with minimal interference from structural analogs and environmental ions. Spiked recovery resultants in water and food samples was obtained as 98.398% - 102.306% and relative standard deviations below 15%, demonstrating reliable accuracy for real-world applications. The proposed electrochemical sensor offered a low-cost, easy-to-fabricate platform for thiamethoxam detection with high sensitivity.

A novel electrochemical sensor based on NiO/MoS2/rGO composite material for rapid detection of methyl parathion

2025-06-23

Chong Tan , Xueyan Liu , Kaijie Yang +3 more | Microchimica Acta

A Sensitive Epinephrine Sensor Based on Photochemically Synthesized Gold Nanoparticles

2025-06-23

Eyup Metin , Gonul S. Batibay , Meral Aydın +1 more | Chemosensors

In this study, gold nanoparticles (AuNPs) and AuNPs-graphene oxide (AuNPs@GO) nanostructures were synthesized in aqueous media using an in-situ photochemical method with bis-acyl phosphine oxide (BAPO) photoinitiator as a photoreducing … In this study, gold nanoparticles (AuNPs) and AuNPs-graphene oxide (AuNPs@GO) nanostructures were synthesized in aqueous media using an in-situ photochemical method with bis-acyl phosphine oxide (BAPO) photoinitiator as a photoreducing agent in the presence of HAuCl4. The parameters for synthesis were arranged to obtain stable and reproducible dispersions with desirable chemical and optical properties. Both AuNPs and AuNPs@GO were employed as sensing platforms for the detection of epinephrine in two concentration ranges: micromolar (µM) and nanomolar (nM). Field emission scanning electron microscopy (FE-SEM), Dynamic Light Scattering (DLS), UV-Vis absorption, fluorescence emission, and Fourier Transform Infrared (FT-IR) spectroscopy techniques were used to investigate the morphological, optical, and chemical properties of the nanostructures as well as their sensing ability towards epinephrine. Fluorescence spectroscopy played a crucial role in demonstrating the high sensitivity and effectiveness of these systems, especially in the low concentration (nM) range, confirming their strong potential as fluorescence-based sensors. By constructing calibration curves on best linear subranges, limit of detection (LOD) and limit of quantification (LOQ) were calculated with two different approaches, SEintercept and Sy/x. Among all the investigated nanostructures, AuNPs@GO exhibited the highest sensitivity towards epinephrine. The efficiency and reproducibility of the in-situ photochemical AuNPs synthesis approach highlight its applicability in small-molecule detection and particularly in analytical and bio-sensing applications.

Iron-Oxide Nanoparticle-Decorated Etched Carbon Nanotube Catalysts for Highly Sensitive Electrochemical Detection of Cholesterol and High-Density Lipoprotein (HDL)

2025-06-23

Sundara Ramaprabhu , Dipsikha Ganguly , Anamika Ghosh +1 more | Journal of The Electrochemical Society

Abstract Cholesterol and high-density lipoprotein (HDL) are very important biomarkers indicative of various health factors in the body. Considering the importance of these analytes in the detection of several diseases, … Abstract Cholesterol and high-density lipoprotein (HDL) are very important biomarkers indicative of various health factors in the body. Considering the importance of these analytes in the detection of several diseases, majorly cardiovascular diseases, it is desirable to develop rapid, sensitive, and selective sensors for their detection. Current reports on sensors for cholesterol and HDL sensing are very limited, and research on them is still in its infancy. In this work, we develop novel cholesterol and HDL sensors based on iron oxide-decorated etched carbon nanotubes-modified screen-printed carbon electrodes synthesized by a simple, cost-effective approach. Suitable enzymes were also immobilized on the electrodes for comparison of the enzymatic sensing performance. The best sensor exhibited a sensitivity of 0.93 μAμM-1cm-2 in the range of 90 – 1080 μM with an LOD of 64.17 μM for cholesterol (non-enzymatic) and a sensitivity of 2136 μAmM-1cm-2 in the range of 0.6 – 1.2 mM with an LOD of 0.2 mM for HDL (enzymatic). The sensor performance was also validated in human serum samples.

Highly efficient non-enzyme glucose sensing by multi-component CuCoNiFeSi alloy with feather-like CuxO

2025-06-23

Jianwu Wen , Shuangqin Chen , Xindong Zhu +6 more | Rare Metals

Recent Trends in Hydrogel-Based Biosensor Technology for the Diagnosis of Neurodegenerative Diseases

2025-06-22

Seda Ayçiçek , Şule Çoşkun Cevher , Selim Acar | ACS Applied Bio Materials

Early diagnosis of neurodegenerative diseases is essential for the development of appropriate treatment strategies. Current diagnostic methods have a number of limitations and challenges. Alternative biomaterials such as hydrogels are … Early diagnosis of neurodegenerative diseases is essential for the development of appropriate treatment strategies. Current diagnostic methods have a number of limitations and challenges. Alternative biomaterials such as hydrogels are potential candidates that are attracting attention in the design process of biosensors for the diagnosis of neurodegenerative diseases. Three-dimensional networks of cross-linked hydrophilic polymers, known as hydrogels, have characteristics that are comparable to those of biological tissues. The integration of hydrogels into biosensor designs for the diagnosis of neurodegenerative diseases offers functionality and structural advantages. This review summarizes recent trends in hydrogel-based biosensors for the detection of neurodegenerative diseases, their design, applications, as well as a comprehensive overview of their advantages and disadvantages.

Agricultural Waste-Based Activated Carbon for Oily Wastewater Treatment by Adsorption: A Review

2025-06-22

Saeid Asadi , Fouad Fadhil Al‐Qaim | Journal of Petroleum Research and Studies

Oily wastewater poses great risks to the ecosystem, human health and living organisms. Therefore, the development and study of sorbents using various activation techniques to remove oils from water resources … Oily wastewater poses great risks to the ecosystem, human health and living organisms. Therefore, the development and study of sorbents using various activation techniques to remove oils from water resources is of interest to the scientific community. Here, we present a review on the use of sorbents made from agricultural waste to remove oils and hydrocarbons from aqueous media. After modification, it was found that activated carbon made from agricultural waste improves the performance of the adsorption process. The presentation also includes a comprehensive analysis of the application of isothermal models, kinetic models and thermodynamic parameters. The selectivity of the sorbent is the main focus of the adsorption kinetics and isothermal models. The process of adsorption and other parameters affecting adsorption such as contact time, solution temperature, solution pH and amount of sorbent are also studied. The use of agricultural waste as sorbents is economically and environmentally beneficial for the removal of oily pollutants.

Early Warning of Drug-Induced Cardiotoxicity: Quantitative Analysis of Mfn2 Biomarker via Electrochemical Immunosensing Technology

2025-06-20

Zixia Wang , Diaoguo Li , Mian Chen +7 more | Research Square (Research Square)

<title>Abstract</title> Drug-induced cardiotoxicity (DIC) poses a significant challenge in both drug development and clinical practice, making early and accurate assessment crucial. Existing studies have shown that mitochondrial fusion mediated by … <title>Abstract</title> Drug-induced cardiotoxicity (DIC) poses a significant challenge in both drug development and clinical practice, making early and accurate assessment crucial. Existing studies have shown that mitochondrial fusion mediated by mitochondrial fusion protein 2 (Mfn2) is closely associated with DIC, and that up-regulation of Mfn2 reduces drug-induced cardiomyocyte damage and apoptosis, suggesting that Mfn2 could be a potential biomarker for early warning assessment of DIC. Due to the defects of cumbersome operation, limited sensitivity, high cost, and difficulty in popularization of existing detection technologies, this study has developed a novel electrochemical immunosensor for highly sensitive detection of Mfn2, enabling early risk assessment of DIC. The sensor utilizes a composite material consisting of sodium titanate nanorods (prepared via MXene oxidation and alkalization) and multi-walled carbon nanotubes (M-NTO-MWCNT) as the sensing substrate. The M-NTO component, with its unique nanorod structure, abundant active sites, and high surface area, significantly enhances sensitivity and provides ample antibody immobilization sites. Meanwhile, MWCNTs improve electron transfer efficiency and selectivity due to their superior conductivity and interconnected network. Under optimized conditions, the sensor achieves a detection limit as low as 1.85 ng mL− 1and a linear range of 9.38×10− 1–2.40×102 ng mL⁻¹. Serum sample testing demonstrated excellent reproducibility (RSD < 5%), outperforming conventional ELISA methods. This study provides a new rapid and portable test solution for the early warning of drug-derived cardiotoxicity, and provides technical support and scientific reference for the safety assessment of new drug development.

2041-LB: Continuous Protein Sensor for Sarcopenia Management during GLP-1RA Therapy

2025-06-20

Rebecca Gottlieb , Bo Wang , Jonathan Kavner +2 more | Diabetes

Introduction and Objective: Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) are highly effective in both weight loss and glycemic control for people with Obesity and/or Diabetes but can result in sarcopenia … Introduction and Objective: Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) are highly effective in both weight loss and glycemic control for people with Obesity and/or Diabetes but can result in sarcopenia - loss of lean muscle mass (LLMM) (1). LLMM effects (2) can be overcome through exercise and increased protein consumption. Phenylalanine (phe) is an essential amino acid released from skeletal muscle breakdown and exogenous protein ingestion. A wearable phenylalanine sensor with activity monitor could track LLMM and protein ingestion for use with these transformative medications. This sensor utilizes a new methodology that relies on an engineered phe bioreceptor using a short nucleic acid sequence (aptamer) labeled with a methylene blue redox probe. This aptamer is attached to an electrode surface where the binding and concentration of the phe is measured through the electrochemical technique square wave voltammetry. Methods: The aptamer bioreceptor was applied to microneedle electrodes and a calibration (0-1500 µM/L) was performed in phosphate buffer solution (PBS). Results: The phe sensor showed log-linear calibration on day 1 and 7 (Fig 1), R^2 d1: 0.986, d7: 0.994, with low limit of detection d1: 3.9 µM/L, d7: 6.4 µM/L. Conclusion: This work demonstrated performance of a proof-of-concept continuous protein monitor utilizing an engineered bioreceptor on a microsensor array that could be used in conjunction with GLP-1 RA therapy. Disclosure R. Gottlieb: Employee; Biolinq. B. Wang: Employee; Biolinq. J.E. Kavner: Employee; Biolinq. K. Mallires: Employee; Biolinq. J.R. Tangney: None.

Metformin Degradation by Advanced Oxidation Processes: Performance, Limitations, and Environmental Concerns

2025-06-20

Jaime M. Castañeda-Sánchez , Felipe de J. Silerio-Vázquez , Ignacio Villanueva-Fierro +3 more | International Journal of Molecular Sciences

This review provides a descriptive analysis of metformin, highlighting its environmental presence and classification as an emerging contaminant. It examines the risks associated with metformin and evaluates advanced oxidation processes … This review provides a descriptive analysis of metformin, highlighting its environmental presence and classification as an emerging contaminant. It examines the risks associated with metformin and evaluates advanced oxidation processes (AOPs) for its degradation, including photolysis, photocatalysis, electrolysis, and ozonation. Metformin, a widely used biguanide for type 2 diabetes, is increasingly detected in aquatic environments due to its incomplete metabolism in humans, raising ecological concerns. While certain AOPs, such as ultraviolet (UV) photocatalysis and ozonation, achieve high degradation rates of 99.9% and 100%, respectively, they produce toxic by-products harmful to aquatic systems. Solar photocatalysis, despite a lower degradation rate (74.22%), stands out for operating without artificial energy and generating fewer hazardous by-products. The review identifies gaps in current degradation strategies and underscores the need for clean, sustainable methods. Future research directions include advancing biological and photocatalytic technologies to improve AOPs’ efficiency while minimizing environmental risks.

A Low-Cost and Environmentally Friendly Electrochemical Biosensor for the Determination of Estradiol

2025-06-20

Cecylia Wardak , Hubert Wólczyński , Szymon Malinowski +2 more | Materials

Estradiol is a natural estrogen belonging to the group of natural steroid hormones. This paper presents new electrochemical biosensors—simple and low-cost tools for the determination of β-estradiol. The receptor layer … Estradiol is a natural estrogen belonging to the group of natural steroid hormones. This paper presents new electrochemical biosensors—simple and low-cost tools for the determination of β-estradiol. The receptor layer of the sensor is the enzyme laccase, which was immobilized on the substrate surface using the soft plasma polymerization technique. This technique is innovative and environmentally friendly as it allows for the effective deposition of the enzyme onto unmodified and modified electrode substrates. Three types of substrates were used: an unmodified glassy carbon electrode and two electrodes modified with composite layers—multi-walled carbon nanotubes combined with CuO nanoparticles and multi-walled carbon nanotubes combined with carbon nanofibers, respectively. Biosensors modified with such materials have not been described previously. In the course of the study, electrochemical measurement conditions (composition, concentration and pH of the base electrolyte, sensor response time, and interference effects) were optimized, and sensor parameters were determined. It was found that the modification of the substrate electrode increased the sensitivity of the sensor by more than 25 times in both cases and led to a lower detection limit for the sensor modified with the carbon nanotubes/carbon nanofiber composite. The best performance was achieved with the sensor containing the carbon nanotube/carbon nanofiber composite layer, which showed a linearity range of 0.1–5 µM, a sensitivity of 7.32 ± 0.22 µA/µM, and a limit of quantification of 0.078 µM. The analytical utility of this biosensor was confirmed by its successful application in the determination of estradiol in pharmaceutical preparations and river water samples.

Affordable and sensitive dual-system electrochemical detection of organophosphorus pesticide residues based on hybridized lipase/AuNPs sensor

2025-06-19

Linlin Chen , Songyao Wu , Li Wei +5 more | Microchemical Journal

Optical Density in Biochemistry: Principles and Applications in Analytical Methods: A Review

2025-06-19

Ali A. Al-fahham , Н. Аббас , Muslim Mukdad Jassim +1 more | International Journal of Medical Science and Dental Health

Optical density is one of the great principles of optics, expressed as the attenuation of light by a medium through which it passes. In most applications, it provides a critical … Optical density is one of the great principles of optics, expressed as the attenuation of light by a medium through which it passes. In most applications, it provides a critical link between the phenomena of light-matter interactions observed in such diverse fields as photonics, materials science, and nanotechnology. It therefore becomes a key parameter in biochemical analysis that links sample absorbance characteristics at given wavelengths quantitatively to desirable determinations for an array of laboratory applications. The measurement of optical density has recently been reported to become central to enzymatic assays with avenues opened into enzyme kinetics, reaction dynamics, and even biomarker detection. The fact that this method is non-invasive places it among methods whose assays have enhanced sensitivity and specificity possible both in clinical diagnostics and biochemical research. This paper presents a review toward synthesis based on recent investigations related explicitly to principles development with implications and applications thereof. It also brings together recent discoveries about how to use optical density in biochemical tests, mainly looking at its part in improving the sharpness and success of many checks and sensor tools. It also pulls together recent clues about using optical density in enzyme tests, points out missing pieces of what we know, and offers where future studies should go. Even with the progress made in this area there are still gaps in our understanding especially about how to link optical density with new technologies and materials.

Development of a ZIF-8/LIG-based electrochemical biosensor for accurate and reliable glucose monitoring in blood

2025-06-19

Xia Kuang , Guodong Zhao , Jingyi Guo +3 more | Microchimica Acta

Ultrasensitive cortisol detection using Ag@Au SERS sensor with molecularly imprinted polymers

2025-06-19

Yuyu Peng , X.H. Zhang , Chunyan Wang +5 more | Sensors and Actuators B Chemical

Analysis of Melatonin Using a Poly(L-Tyrosine)-Modified Graphene Paste Electrode

2025-06-19

D. Sumanth , J. G. Manjunatha , Tahani Mazyad Almutairi +1 more | Journal of Electronic Materials

Simultaneous determination of epinephrine and folic acid using MIL-101 (Fe)-NH2 metal-organic framework/graphene oxide nanocomposite modified electrode

2025-06-19

Rasha Kareem Khudhur | ADMET & DMPK

Background and purpose: It is generally known that the majority of disorders exhibit symptoms to some degree when the quantities of two crucial substances, epinephrine and folic acid, are low … Background and purpose: It is generally known that the majority of disorders exhibit symptoms to some degree when the quantities of two crucial substances, epinephrine and folic acid, are low or high. These two chemicals' composition variations may be tracked and utilized to identify conditions such as myocardial infarction, Parkinson's disease, and mental disorders. Experimental approach: Using a solvothermal technique, we propose the synthesis of a novel MIL-101 (Fe)-NH2 metal-organic framework/graphene oxide nanocomposite (MOF/GO nanocomposite). The produced nanocomposite's morphology was examined using field-emission scanning electron microscopy. A straightforward, quick, and sensitive electrochemical sensing platform for epinephrine detection was then created by drop-casting the produced MOF/GO nanocomposite onto the screen-printed electrode (SPE). Key results: Compared to unmodified SPE, cyclic voltammetry revealed that the MOF/GO/SPE considerably enhanced the epinephrine oxidation process, exhibiting a greater detection current at a lower over-potential. The synergistic combination of MOF and GO sheets may cause this discovery. With a low detection limit of 0.07 μM, the MOF/GO/SPE sensor's linear response for voltammetric measurements of epinephrine was found to be between 0.2 and 500.0 μM. A modified electrode was also utilized to measure folic acid and epinephrine simultaneously. Conclusion: Lastly, the modified SPE effectively demonstrates its high accuracy in identifying folic acid and epinephrine in biological and pharmaceutical samples.

Synthesis of Nickel Oxide Nanomaterials for Electrochemical Detection of Glucose in Different Alkaline Environments

2025-06-19

Runhua Qin , Jiawen Li , Jibin Zhou +2 more | Journal of Electronic Materials

Magnetic bismuth sulfide-decorated activated carbon nanocomposite for ultrasensitive electrochemical determination of felodipine in real samples

2025-06-19

Mohamed Abd-Elsabour , Mortaga M. Abou‐Krisha , Abdulrahman G. Alhamzani +2 more | Diamond and Related Materials

AI-Enhanced Glucose Detection Using a Circular based SPR Biosensor with Graphene-Mxene-Au-Architecture

2025-06-19

S. Vidhya , Jacob Wekalao , Ganta Raghotham Reddy | Plasmonics

Protein-Enhanced Photoelectrochemical Sensor for Antioxidant Detection in Biodiesel Using Zinc Vanadate and Sulfur-Doped Graphitic Carbon Nitride

2025-06-19

Chirlene Nascimento Botelho , Jefferson Santos Oliveira , Adeílton Pereira Maciel +3 more | ACS Omega

Dual-mode biosensor for monoamine oxidase B activity detection based on electrochemistry and surface-enhanced Raman scattering

2025-06-18

Zhenxing Jiang , Chenchen Wang , Xiaolei Song +2 more | Analytica Chimica Acta

Cobalt metaphosphate nanoparticles encapsulated in pomelo peel-derived N,P co-doped porous biochar for enhanced electrochemical sensing of dopamine

2025-06-18

Xinxi Li , Li Xu , Junchun Guo +2 more | Microchemical Journal

Ag nanoparticle decorated NiAl LDH/rGO nanocomposites modified probe towards electrochemical detection of dopamine in real samples

2025-06-18

Praveena Prabhakaran , Biny R. Wiston , M. Ashok | Journal of Alloys and Compounds

Enhanced electrochemical nitrite sensing based on a gold nanoparticle decorated two-dimensional reduced graphene oxide/graphitic carbon nitride nanocomposite

2025-06-18

Qingfang Niu , Xiuying Peng , Junhua Meng +5 more | Microchimica Acta

Generation and Nitric Oxide Reactivity of a Cobalt(II) Superoxide Complex via Guanidine-Based Ligand Non-Innocence

2025-06-18

Dibya Jyoti Barman , Thomas Lohmiller , Konstantin B. Krause +4 more | JACS Au

Target-responsive vesicular nanoprobe for fluorescent detection of lipase

2025-06-18

Sixuan Cheng , Xiaomeng Zheng , Ziming Li +6 more | Microchimica Acta

Gluconeogenesis in Aqueous Microdroplets: Non-Enzymatic Generation of Glucose

2025-06-18

Jeong-Ae Ko , Yu‐Kyung Kim , Jeongcheol Lee +1 more | bioRxiv (Cold Spring Harbor Laboratory)

Abstract Glucose is a central metabolite of living organisms, serving as the primary energy substrate produced predominantly by photosynthetic organisms. Under conditions of limited external glucose supply, organisms activate gluconeogenesis, … Abstract Glucose is a central metabolite of living organisms, serving as the primary energy substrate produced predominantly by photosynthetic organisms. Under conditions of limited external glucose supply, organisms activate gluconeogenesis, an endogenous biosynthetic pathway that sustains essential glucose levels. Yet the mechanism of glucose generation, before the advent of photosynthesis and complex enzymatic systems, remains elusive. Recently, microdroplet chemistry has emerged as a novel approach for catalyst-free organic synthesis. Here, we report the non-enzymatic formation of glucose from a simpler organic precursor, pyruvate, in aqueous microdroplets without the aid of organic or inorganic catalysts. Our results show that glucose is generated via a reaction pathway analogous to canonical gluconeogenesis, proceeding through key intermediates including oxaloacetate, glycerate, and glyceraldehyde. Furthermore, thermodynamic analysis indicates that the free energy change associated with glucose formation is overcome in aqueous microdroplets at room temperature, without the need for external energy input or enzymatic catalysis. These findings indicate that aqueous microdroplets can non-enzymatically convert C 3 compound, pyruvate, into the C 6 sugar glucose, offering a plausible abiotic route for anabolic carbon transformations during abiogenesis.

Sulfur-inspired layered g-C3N4-incorporated NiFe2S4 nanocomposite for electrochemical and real-time detection of vanillin in food samples

2025-06-18

A. Tamilselvan , M G Gopika , Aravinth Karuppanan +3 more | Journal of the Taiwan Institute of Chemical Engineers

A photoelectrochemical sensing based on the ternary metal sulfide FeIn2S4@MoS2/CdS heterojunction for miniaturization design of dopamine detection

2025-06-18

Shunyou Wang , Huafei Yi , Xiang Yu +2 more | Microchemical Journal

A green solid-phase spectrophotometric method for Fe(III) detection based on immobilised methyl thymol blue from wastewater

2025-06-18

Ra’no Norboyeva , Zulayho Smanova , Samariddin Rakhimov +7 more | International Journal of Environmental & Analytical Chemistry

Ultrasensitive Quantification of Thyroid-Stimulating Hormone and Thyroxine by Nanoelectronic SnS2 Transistor Sensors

2025-06-17

Ankur Anand , Feng-Yi Su , Tse-Hao Chen +2 more | ACS Sensors

The measurement of thyroid hormones in serum is widely regarded as the most valuable single laboratory tool for assessing thyroid function. This study presents a highly sensitive tin disulfide nanosheet-fabricated … The measurement of thyroid hormones in serum is widely regarded as the most valuable single laboratory tool for assessing thyroid function. This study presents a highly sensitive tin disulfide nanosheet-fabricated field-effect transistor (SnS2-FET) designed for the detections of human thyroid-stimulating hormone (hTSH) and thyroxine (T4). By co-modifying an antibody (AbTSH for detecting hTSH), or a DNA aptamer (AptT4 for detecting T4), with polyethylene glycol (PEG) on the SnS2-FET channel surface, the PEG:AbTSH/SnS2-FET and PEG:AptT4/SnS2-FET devices achieve highly sensitive and selective detections of hTSH and T4, respectively, even in a high ionic strength buffer (1× PBS) or undiluted serum. With a low limit of detection (in the femtomolar level) and a wide linear working range (spanning at least 6 orders of magnitude of analyte concentration), the PEG:AbTSH/SnS2-FET immunosensor and PEG:AptT4/SnS2-FET aptasensor can detect the hTSH and T4 levels encountered in the spectrum of thyroid disorders. Notably, these specific receptor-modified SnS2-FET devices display negligible cross-reactivity with other pituitary hormones or serum components. This research indicates that the nanoelectronic SnS2-FET sensor platforms hold significant potential for point-of-care clinical diagnostics, particularly for the ultrasensitive detection and early screening of medical conditions.

Laccase-modified graphene electrodes obtained by direct laser writing for the sensitive detection of dopamine in real samples

2025-06-17

Bibata Ouedraogo , Amidou Tall , Sabrine Baachaoui +2 more | Emergent Materials

Development of a novel electrochemical sensor for determination of acetaminophen in the presence of 4-aminophenol using Ni-MOF nanosheets/GO nanocomposite-modified glassy carbon electrode

2025-06-17

Asma Sedrizadeh , Niloufar Akbarzadeh‐T , Somayeh Tajik +1 more | Monatshefte für Chemie - Chemical Monthly

Enzymatic biofuel cell on flexible nanoporous gold electrodes

2025-06-17

Denise Demurtas , Julia Álvarez-Malmagro , Andrés Felipe Quintero-Jaime +7 more | Bioelectrochemistry

Application of Non-G-Quadruplex Hemin Aptamers to Hemin Detection and Heme Oxygenase 1 Activity Evaluation via Spatial Conformational Constraint

2025-06-17

Changjiang Li , Jiani Wu , Xiaolong Li +1 more | Analytical Chemistry

The correct spatial folding of the aptamer significantly influences its binding performance with the target. By extending the terminal double-stranded domain of the hemin aptamer, we obtained apt71 with a … The correct spatial folding of the aptamer significantly influences its binding performance with the target. By extending the terminal double-stranded domain of the hemin aptamer, we obtained apt71 with a more stable spatial conformation. The catalytic activity of the resulting apt71/hemin DNAzyme reached 200% of that of the original aptamer. We systematically evaluated the aptamer's performance in terms of apparent dissociation constant, average catalytic efficiency, and substrate affinity. It was successfully applied to the visual detection of hemin, achieving a detection limit of 0.1 nM, with detection performance comparable to previously developed fluorescence and chemiluminescence strategies. High-sensitivity visual detection was demonstrated in both diluted serum and environmental water samples, along with high specificity in distinguishing multiple hemin analogs. Furthermore, the apt71/hemin DNAzyme system was applied to the in vitro activity evaluation of heme oxygenase 1, compared to traditional G-quadruplex aptamers, our optimized non-G-quadruplex hemin aptamer exhibited improved performance, confirming its significant practical value and broad application potential in hemin-related research.

Advances in Electrochemical Detection of Vitamins B2 and B6: A Brief Review on Practical Applications

2025-06-17

M G Gopika , C. Raril , Bhama Sajeevan | Critical Reviews in Analytical Chemistry

The precise measurement of vitamins B2 (VB2) and B6 (VB6) is of great concern for clinics, pharmacists, and food manufacturers, as these vitamins are of great importance and cannot be … The precise measurement of vitamins B2 (VB2) and B6 (VB6) is of great concern for clinics, pharmacists, and food manufacturers, as these vitamins are of great importance and cannot be made by the body. Undoubtedly, the reliable quantification of these vitamins is a great analytical problem considering the serious metabolic disorders that can occur because of their deficiencies. Conventional HPLC, fluorescence spectroscopy, and capillary electrophoresis are highly accurate methods, but due to high costs, intricate sample preparation, and slow analysis times, their application is quite limited. The introduction of electrochemical sensing was a much-needed alternative as its simplicity, cost-effectiveness, high sensitivity, and fast response is desired within the monitoring field. Advances in the electrochemical techniques, and particularly in the voltammetric methods, as well as their electrode materials and surface modifications have improved the simultaneous detection of these vitamins. Nonetheless, sensor selectivity, reproducibility, and signal interference are significant hurdles for their practical implementation. In this review, we describe the importance of the electrochemical sensors in VB2 and VB6 detection, the current state of these sensors and their limitations, and the prospective future development work to improve their performance.

Hydrothermally Grown p-Type CuO Nanotaper for Saliva Glucose Sensing Application

2025-06-17

Tanmoy Majumder , Kaberi Saha , Kamalesh Debnath +2 more | Electrocatalysis